Inhibition of Kupffer Cell Activity Improves Transplantation of Human Adipose-Derived Stem Cells and Liver Functions

Cell Transplantation

Article

Kyung-Sun Kang¹  Mi-Ran Ki²  Il-Hwa Hong²  Jin-Kyu Park²  Eun-Mi Lee³  Sang-Young You³  Ah-Young Kim³  Kyu-Shik Jeong³  Seon-Young Han³  Eun-Joo Lee³  Young-Mi Moon⁴  Jae-Ho Jeong⁴ 

[1] Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea;College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea;College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea;Stem Cell Therapeutic Research Institute, Kyungpook National University, Daegu, Republic of Korea;Division of Plastic Surgery, Yeungnam University Medical Center, Daegu, Republic of Korea;

关键词: Adipose-derived stem cells (ASCs);    Glycine;    Kupffer cells;    Liver;    Tumor necrosis factor-α (TNF-α);   

DOI  :  10.3727/096368912X640583

 received in 2011-07-26, accepted in 2012-03-27,  发布年份 2013

来源: Sage Journals

【 摘 要 】

Numerous approaches to cell transplantation of the hepatic or the extrahepatic origin into liver tissue have been developed; however, the efficiency of cell transplantation remains low and liver functions are not well corrected. The liver is a highly immunoreactive organ that contains many resident macrophages known as Kupffer cells. Here, we show that the inhibition of Kupffer cell activity improves stem cell transplantation into liver tissue and corrects some of the liver functions under conditions of liver injury. We found that, when Kupffer cells were inhibited by glycine, numerous adipose-derived stem cells (ASCs) were successfully transplanted into livers, and these transplanted cells showed hepatoprotective effects, including decrease of liver injury factors, increase of liver regeneration, and albumin production. On the contrary, injected ASCs without glycine recruited numerous Kupffer cells, not lymphocytes, and showed low transplantation efficiency. Intriguingly, successfully transplanted ASCs in liver tissue modulated Kupffer cell activity to inhibit tumor necrosis factor-α secretion. Thus, our data show that Kupffer cell inactivation is an important step in order to improve ASC transplantation efficiency and therapeutic potential in liver injuries. In addition, the hepatoprotective function of glycine has synergic effects on liver protection and the engraftment of ASCs.

【 授权许可】

Unknown   
© 2013 Cognizant Comm. Corp.

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