Mature Adipocyte-Derived Cells, Dedifferentiated Fat Cells (DFAT), Promoted Functional Recovery from Spinal Cord Injury-Induced Motor Dysfunction in Rats

Cell Transplantation

Article

Koichiro Kano¹  Hideyuki Okano²  Taro Matsumoto³  Hideo Mugishima³  Yukie Tokura⁴  Rie Igarashi⁴  Mitsuko Takenaga⁴  Yuki Ohta⁴  Akemi Hamaguchi⁴ 

[1] Department of Animal Sciences, College of Bioresource Sciences, Nihon University, Fujisawa 252-8510, Japan;Department of Physiology, Keio University School of Medicine, Tokyo 160-8582, Japan;Division of Cell Regeneration and Transplantation, Advanced Medical Research Center, Nihon University School of Medicine, Tokyo 173-8610, Japan;Institute of Medical Science, St. Marianna University School of Medicine, Kawasaki 216-8512, Japan;

关键词: Mature adipocytes;    Dedifferentiated fat cells (DFAT);    Spinal cord injury;    Transplantation;    Neurotrophic factor;   

DOI  :  10.3727/096368908786576516

 received in 2007-05-10, accepted in 2008-02-12,  发布年份 2008

来源: Sage Journals

【 摘 要 】

Transplantation of mature adipocyte-derived cells (dedifferentiated fat cells) led to marked functional recovery from spinal cord injury (SCI)-induced motor dysfunction in rats. When mature adipocytes were isolated from rat adipose tissue and grown in ceiling culture, transformation into fibroblast-like cells without lipid droplets occurred. These fibroblast-like cells, termed dedifferentiated fat cells (DFAT), could proliferate and could also differentiate back into adipocytes. DFAT expressed neural markers such as nestin, βIII tubulin, and GFAP. Allografting of DFAT into SCI-induced rats led to significant recovery from hindlimb dysfunction. Grafted cells were detected at the injection site, and some of these cells expressed βIII tubulin. DFAT expressed neurotrophic factors such as BDNF and GDNF prior to transplantation, and grafted cells were also positive for these factors. Therefore, these neurotrophic factors derived from grafted DFAT might have contributed to the promotion of functional recovery. These findings also suggest that mature adipocytes could become a new source for cell replacement therapy to treat central nervous system disorders.

【 授权许可】

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