Tongue Cancer and Epigenetic Factors: An in-vitro Study on 298 Micro-RNAS

European Journal of Inflammation

Original Article

C. Sbordone¹  D. Pasquali²  A. Santoro³  G. Pannone³  P. Bufo³  L. Lo Muzio⁴  G. Russo⁵  A. Guida⁶  R. Serpico⁶  A. Lucchese⁶  S. Papagerakis⁷  G. Donnarumma⁸ 

[1] Chair of Maxillo-Facial Surgery, School of Medicine, University of Naples Federico II, Naples, Italy;Department of Clinical and Experimental Medicine and Surgery, Endocrine Unit, Second University of Naples SUN, Naples, Italy;Department of Clinical and Experimental Medicine, Section of Anatomic Pathology, University of Foggia, Foggia, Italy;Department of Clinical and Experimental Medicine, Section of Oral Pathology, University of Foggia, Foggia, Italy;Department of Clinical and Experimental Medicine, University of Foggia, Foggia, Italy;Department of Odontostomatological, Orthodontics and Surgical Disciplines, Second University of Naples SUN, Naples, Italy;Department of Otolaryngology, Head and Neck Surgery Laboratory of Oral, Head and Neck Cancer Invasion and Metastasis, Medical School, University of Michigan Ann Arbor, Ann Arbor, MI, USA;Second University of Naples, Department of Experimental Medicine, Microbiology and Clinical Microbiology, Naples, Italy;

关键词: Oral squamous cell carinoma;    OSCC;    tongue cancer;    tongue squamous cell carcinoma;    epigenetics;    micro-RNA;    miRNA;    CDKN2a;    p16;    p53;    SCC-4;    SCC-15;    SCC-25;   

DOI  :  10.1177/1721727X1201000320

 received in 2012-04-05, accepted in 2012-08-31,  发布年份 2012

来源: Sage Journals

【 摘 要 】

Oral Squamous Cell Carcinoma (OSCC) is the sixth most frequent malignant tumour. There is some evidence that tongue cancer has a higher local failure rate and poorer prognosis than other anatomical sites in the oral cavity. We used tongue squamous cell carcinoma cell lines harbouring mutated p53/p16 as tongue cancer models to study the influences exerted by p53 and p16 genes on the expression of micro RNAs (miRNAs). The study was performed on microarray chips harbouring 298 miRNA sequences. OSCC cell lines used in this study were SCC-4, SCC-15 and SCC-25, all three carrying mutated/hypermethylated p53/p16. The expression values normalized to healthy control of 298 miRNAs were obtained for each cell line. MiRNA 196b was found hyperexpressed in the three cell lines. MiRNAs 19b-1, 21, 27a, 30d, 134, 339, 379 and 465 were found altered in two out of three cell lines. miRNAs found altered in one cell line out of three were: 7b, 23a, 25, 30c, 30e-3p, 107,125b, 124a, 214, 216, 325 and 384. A literature review for each miRNA found significant was undertaken. Some miRNAs have a well-known role in oral cancer, some have been put in correlation with other cancers/diseases, others are found significant for the first time. These early results in tongue cancer cell lines harbouring mutation of p16/p53 need further analyses to understand whether this variation of miRNA levels are directly influenced by the malfunction of these proteins or if, vice-versa, altered miRNA levels influence the function of p16 and p53.

【 授权许可】

Unknown   
© 2012 SAGE Publications