Intravenous Administration of Human Umbilical Cord Blood Reduces Neurological Deficit in the Rat after Traumatic Brain Injury

Cell Transplantation

Article

Juan Sanchez-Ramos¹  Paul R. Sanberg²  Lei Wang³  Yi Li³  Michael Chopp⁴  Asim Mahmood⁵  Dunyue Lu⁵ 

[1] Center for Aging and Repair, Departments of Neurology, University of South Florida, Tampa, FL;Center for Aging and Repair, Departments of Neurosurgery, University of South Florida, Tampa, FL;Departments of Neurology, Henry Ford Health Sciences Center, Detroit, MI;Departments of Neurology, Henry Ford Health Sciences Center, Detroit, MI;Department of Physics, Oakland University, Rochester, MI;Departments of Neurosurgery, Henry Ford Health Sciences Center, Detroit, MI;

关键词: Traumatic brain injury (TBI);    Rat;    Human umbilical cord blood (HUCB);    Intravenous administration;   

DOI  :  10.3727/096020198389924

来源: Sage Journals

【 摘 要 】

We measured the effect of treatment of traumatic brain injury (TBI) in the rat with human umbilical cord blood (HUCB) administered IV. HUCB cells were injected into the tail vein 24 h after TBI and the rats were sacrificed at day 28 after the treatment. The Rotarod test and the neurological severity score (NSS) were used to evaluate neurological function. The distribution of the donor cells in the brain, heart, lung, kidney, liver, spleen, bone marrow, and muscle were analyzed in recipient rats using immunohistochemical staining and laser confocal microscopy. HUCB cells injected IV significantly reduced motor and neurological deficits compared with control groups by day 28 after the treatment. The cells preferentially entered the brain and migrated into the parenchyma of the injured brain and expressed the neuronal markers, NeuN and MAP-2, and the astrocytic marker, GFAP. Some HUCB cells integrated into the vascular walls within the boundary zone of the injured area. Our data suggest that IV administration of HUCB may be useful in the treatment of TBI.

【 授权许可】

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