Susceptibility and Resistance to Canine Leishmaniose is Associated to Polymorphisms of the Canine TNF-α Gene

European Journal of Inflammation

Original Article

S. Pappa¹  M. Hatzistilianou¹  A. Kourelis²  C.N. Pantzartzi²  E. Karagouni³  C.I. Dovas⁴  I. Symeonidou⁵  S. Frydas⁵  E. Papadopoulos⁵ 

[1] 2nd Department of Paediatrics, Aristotle University of Thessaloniki, Thessaloniki;Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Thessaloniki, Greece;Laboratory of Cellular Immunology, Institute Pasteur Hellenique, Athens;Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki;Laboratory of Parasitology and Parasitic Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki;

关键词: canine leishmaniosis;    SNPs;    TNF-α;    susceptibility;    resistance;   

DOI  :  10.1177/1721727X1100900104

 received in 2010-07-12, accepted in 2010-09-13,  发布年份 2011

来源: Sage Journals

【 摘 要 】

The prevalence of canine leishmaniosis (CanL) infection in an enzootic area is considerably higher than the overall prevalence of the disease, suggesting a role of host genetics related to the outcome of the disease. It is accepted that one determining factor for the outcome of CanL is the type of the triggered immune response, which seems to be genetically determined. TNF-α is a cytokine which plays a crucial role during the immune response against Leishmania parasites. In the present study a case-control study with 20 resistant and 20 susceptible dogs was performed. The distribution of breeds was equal in both groups. By Sanger method the nucleotide sequence upstream the Open Reading Frame of the canine TNF-α gene was determined and four polymorphisms were identified (−40 C/A, −1134 T/G, −1150 T/C κα −1243 C/G). Statistical analysis showed that the polymorphism TNF-α −40 C/A is correlated with susceptibility to CanL, while the polymorphism TNF-α −1243 C/G is correlated with resistance to CanL. Further statistical analysis, regarding the possible correlation of gender as well as clinical manifestations of the disease with the above-mentioned polymorphisms of the TNF-α gene, showed no significant findings. Further analysis of the above polymorphisms, as well as identification of more polymorphisms in candidate genes, is required to provide a better understanding of the complex underlying immune response in CanL.

【 授权许可】

Unknown   
© 2011 SAGE Publications