Use of Repeating Dispensers to Increase the Efficiency of the Intramuscular Myogenic Cell Injection Procedure

Cell Transplantation

Brief Communication

Marlyne Goulet¹  Daniel Skuk¹  Jacques P. Tremblay¹ 

[1] Unité de recherche en Génétique humaine, Centre Hospitalier de l'Université Laval, Québec, QC, Canada G1V 4G2;

关键词: Cell delivery;    Muscle precursor cell;    Repeating dispensers;    Skeletal muscle;   

DOI  :  10.3727/000000006783981648

 received in 2006-03-22, accepted in 2006-05-08,  发布年份 2006

来源: Sage Journals

【 摘 要 】

Intramuscular myoblast transplantation in humans and nonhuman primates requires precise repetitive cell injections very close to each other. Performed with syringes operated manually throughout large regions, this procedure takes a lot of time, becoming tiring and thus imprecise. We tested two repetitive dispensers with Hamilton syringes as cell injection devices to facilitate this procedure. Monkeys received intramuscular allotransplantations of β-galactosidase-labeled myoblasts, using either a monosyringe or a multisyringe repeating dispenser. The monosyringe repeating dispenser allowed performing cell injections faster and easier than with a manually operated syringe. The multisyringe dispenser accelerated the procedure still more, but it was not ergonomic. Biopsies of the myoblast-injected sites 1 month later showed abundant β-galactosidase-positive myofibers, with the same density and morphological pattern observed following myoblast transplantation with a syringe operated manually. We recommend the monosyringe repeating dispenser for myoblast transplantation in skeletal muscles and maybe in the heart.

【 授权许可】

Unknown   
© 2006 Cognizant Comm. Corp.