Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation

Cell Transplantation

Article

Norio Sakuragawa¹  Shin Enosawa²  Hiroshi Amemiya²  Tasuku Mitani²  Nanae Takahashi²  Hua Lu²  Seiichi Suzuki²  Takashi Amano³ 

[1] National Institute of Neuroscience;National Research Institute for Child Health and Development;Tokyo University of Agriculture;

关键词: Amniotic epithelial cells;    In utero manipulation;    Rat fetus;    Liver;    Gene carrier;   

DOI  :  10.3727/000000002783985602

来源: Sage Journals

【 摘 要 】

It has been hoped that amniotic epithelial cells would be a gene carrier to neural and hepatic tissue, because of 1) the presence of neural and hepatic stem-like cells, 2) the ability to cryopreserve them, 3) long-term survival in the transplanted site, and 4) few ethical problems concerning procurement. But transplantation of a sufficient number of cells to adult tissue needs large-scale cell supply and may lead to vascular embolism. We attempted transplantation of amniotic epithelial cells into fetal liver, because 1) the fetal liver is at the proliferative stage, 2) the number of cells required is small, and 3) the fetal stage is advantageous for the induction of immunological tolerance. Amniotic epithelial cells from day 18.5–20.5 fetuses were transfected with adenoviral AdlacZ and harvested to inject into fetal rat liver of the syngeneic strain (day 18.5–20.5). The efficacy of cell transplantation into the liver increased in the order: intraplacental < intraumbilical vein < intrahepatic route. LacZ-transfected amniotic cells (1–8 × 105 cells), hepatocytes (5 × 105 cells), or AdlacZ vector solution (1.7 × 107 pfu) were injected through the uterine membrane into the liver. Transplanted cells formed a cellular mass and survived for up to 14 days after birth, whereas lacZ-transfected cells were rapidly decreased after the injection of AdlacZ vector or rat hepatocytes as a gene carrier so that the use of amniotic epithelial cells as a gene carrier will result in long-term expression of exogenous genes in the liver.

【 授权许可】

Unknown   
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