Osteogenic differentiation of amniotic epithelial cells: synergism of pulsed electromagnetic field and biochemical stimuli

BMC Musculoskeletal Disorders

Xiaojing Liu⁴  Fengming Luo¹  Chengqi He²  Huaiqing Chen³  Jiang Wu³  Song Lei⁶  Ai Zheng⁵  Xiaoyu Han⁴  Wenchao Wu⁴  Qian Wang² 

[1] Golden Card Ward, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China;Sichuan University-The Hong Kong Polytechnic University Institute for Disaster Management and Reconstruction, Sichuan University, Chengdu 610041, China;Laboratory of Biomedical Engineering, West China Center of Medical Sciences, Sichuan University, Chengdu, Sichuan, P. R. China;Laboratory of Cardiovascular Diseases, Regenerative Medicine Research Center, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China;Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu, Sichuan, P. R. China;Department of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan, P. R. China

关键词: Integrinβ1;    Reactive oxygen species;    Wnt/β-catenin signaling;    BMP-2;    Pulsed electromagnetic field;    Osteogenic differentiation;    Amniotic epithelial cells;   

Others  :  1122228
DOI  :  10.1186/1471-2474-15-271

 received in 2014-02-17, accepted in 2014-06-28,  发布年份 2014

【 摘 要 】

Background

Pulsed electromagnetic field (PEMF) is a non-invasive physical therapy used in the treatment of fracture nonunion or delayed healing. PEMF can facilitate the osteogenic differentiation of bone marrow mesenchymal stem cells in vitro. Amniotic epithelial cells (AECs) have been proposed as a potential source of stem cells for cell therapy. However, whether PEMF could modulate the osteogenic differentiation of AECs is unknown. In the present study, the effects of PEMF on the osteogenic differentiation of AECs were investigated.

Methods

AECs were isolated from amniotic membrane of human placenta by trypsin digestion and were induced by PEMF and/or osteo-induction medium. After 21 days we used real time RT-PCR and immunocytochemistry to study the expression of osteoblast markers. The signal transduction of osteogenesis was further investigated.

Results

The PEMF stimulation, or osteo-induction medium alone could induce osteogenic differentiation of AECs, as shown by expression of osteoblast specific genes and proteins including alkaline phosphatase and osteocalcin. Furthermore, a combination of PEMF and osteo-induction medium had synergy effects on osteogenic differentiation. In our study, the gene expression of BMP-2, Runx2, β-catenin, Nrf2, Keap1 and integrinβ1 were up-regulated in the osteogenic differentiation of AECs induced by PEMF and/or osteo-induction medium.

Conclusions

Combined application of PEMF and osteo-induction medium is synergistic for the osteogenic differentiation of AECs. It might be a novel approach in the bone regenerative medicine.

【 授权许可】

   
2014 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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