Efficient Intracytoplasmic Labeling of Human Umbilical Cord Blood Mesenchymal Stromal Cells with Ferumoxides

Cell Transplantation

Article

Tae Seok Park¹  Soo Yeol Lee¹  Sin-Soo Jeun²  Bum-Soo Kim³  Sung-Eun Yang⁴  Dal-Soo Kim⁴  Yoon Sun Yang⁴  Wonil Oh⁴  Hye Jin Jin⁴  Man Kyoung Lee⁴  Jae Kwon Lee⁵ 

[1] Department of Biomedical Engineering, Kyung Hee University, Seoul, 130-701, Republic of Korea;Department of Neurosurgery, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea;Department of Radiology, College of Medicine, The Catholic University of Korea, Seoul, 137-701, Republic of Korea;Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea;Medipost Biomedical Research Institute, Medipost Co. Ltd., Asan Institute of Life Science, Seoul, 138-736, Republic of Korea; School of Science Education (Biology Education), College of Education, Chungbuk National University, Chungcheongbuk-Do, 361-763, Republic of Korea;

关键词: Umbilical cord blood;    Mesenchymal stromal cells;    Superparamagnetic iron oxide;    Ferumoxides;    Multilineage differentiation;   

DOI  :  10.3727/000000007783465271

 received in 2007-01-12, accepted in 2007-06-05,  发布年份 2007

来源: Sage Journals

【 摘 要 】

Mesenchymal stromal cells (MSCs) are multipotent cells found in several adult tissues; they have the capacity to differentiate into mesodermal, ectodermal, and endodermal tissues in vitro. There have been several reports that MSCs have therapeutic effects in a variety of diseases. Therefore, using a cell labeling technique, monitoring their temporal and spatial migration in vivo, would be useful in the clinical setting. Magnetic resonance imaging (MRI)—tracking of superparamagnetic iron oxide (SPIO)-labeled cells—is a noninvasive technique for determining the location and migration of transplanted cells. In the present study, we evaluated the influence and toxicity of SPIO (ferumoxides) labeling on multiple differentiated MSCs. To evaluate the influence and toxicity of ferumoxides labeling on differentiation of MSCs, a variety of concentrations of ferumoxides were used for labeling MSCs. We found that the cytoplasm of adherent cells was effectively labeled at low concentrations of ferumoxides. Compared with unlabeled controls, the ferumoxides-labeled MSCs exhibited a similar proliferation rate and apoptotic progression. The labeled MSCs differentiated into osteoblasts and adipocytes in an identical fashion as the unlabeled cells. However, chondrogenesis and neurogenesis were inhibited at high concentrations of ferumoxides. Our results suggest the effective concentration for ferumoxides use in tracking MSCs.

【 授权许可】

Unknown   
© 2007 Cognizant Comm. Corp.