Effects of Iron Oxide Nanoparticle Labeling on Human Endothelial Cells

Cell Transplantation

Article

Ming-Xi Yu¹  Wen-Li Chen¹  Fu-Yuan Yang¹  Quan Zhou²  Peng Gao²  Zheng Huang³ 

[1] MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou, China;Medical Imaging Center, the First Affiliated Hospital of Jinan University, Guangzhou, China;School of Medicine and School of Engineering and Applied Science, University of Colorado-Denver, Denver, CO, USA;

关键词: Iron oxide nanoparticles (INOPS);    Magnetic resonance imaging (MRI);    Cell tracking;    Cell labeling;   

DOI  :  10.3727/096368912X652986

 received in 2011-02-21, accepted in 2011-11-11,  发布年份 2012

来源: Sage Journals

【 摘 要 】

Iron oxide nanoparticles (INOPS) are a potential contrast agent for magnetic resonance (MR) tracking of transplanted endothelial cells. The objective of this study was to examine the effect of INOPS labeling on endothelial cells. The mixture of INOPS and poly-l-lysine (PLL) was used to label human endothelial cells. Labeling efficiency was examined by Prussian blue staining, transmission electron microscopy, and atomic absorption spectrometry. The effect of iron oxide concentration on cell viability and proliferation were determined. The correlation of reactive oxygen species (ROS) and apoptosis was also examined. In vitro MRI scanning was carried out using a 1.5T MR system. INOPS-PLL could be readily taken up by endothelial cells and subsequently induce MRI signal intensity changes. However, higher labeling concentration (>50 μg/ml) and longer incubation (48 h) can affect cell viability and proliferation. Mitochondrial damage, apoptosis, and autolysosmes were observed under high INOPS-PLL concentrations, which were correlated to ROS production. INOPS-PLL nanoparticles can be used to label transplanted endothelial cells. However, high concentration of INOPS can impair cell viability, possibly through ROS-mediated apoptosis and autophagy.

【 授权许可】

Unknown   
© 2012 Cognizant Comm. Corp.

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