Comparison of Human Adipose-Derived Stem cells and Bone Marrow-Derived Stem cells in a Myocardial Infarction Model

Cell Transplantation

Article

Jens Kastrup¹  Ulf Simonsen²  Jeppe Grøndahl Rasmussen³  Claus Holst-Hansen⁴  Ole Frøbert⁵  Ulrik Baandrup⁶  Vladimir Zachar⁷  Trine Fink⁷ 

[1] Cardiac Stem Cell Laboratory, The Heart Centre, Rigshospitalet, Copenhagen University Hospital, Copenhagen, Denmark;Department of Biomedicine, Pulmonary and Cardiovascular Pharmacology, Aarhus University, Aarhus, Denmark;Department of Biomedicine, Pulmonary and Cardiovascular Pharmacology, Aarhus University, Aarhus, Denmark;Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark;Department of Cardiology, Head and Heart Centre, Aalborg Hospital, Århus University Hospital, Aalborg, Denmark;Department of Cardiology, Örebro University Hospital, Örebro, Sweden;Department of Pathology, Vendsyssel Hospital, Hjørring, Denmark;Center for Clinical Research, Aalborg University, Aalborg, Denmark;Laboratory for Stem Cell Research, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark;

关键词: Myocardial infarction;    Cell therapy;    Left ventricular function;    Remodeling;    Angiogenesis;   

DOI  :  10.3727/096368912X659871

 received in 2012-05-30, accepted in 2012-11-16,  发布年份 2014

来源: Sage Journals

【 摘 要 】

Treatment of myocardial infarction (MI) with bone marrow-derived mesenchymal stem cells and recently also adipose-derived stem cells has shown promising results. In contrast to clinical trials and their use of autologous bone marrow-derived cells from the ischemic patient, the animal MI models are often using young donors and young, often immune-compromised, recipient animals. Our objective was to compare bone marrow-derived mesenchymal stem cells with adipose-derived stem cells from an elderly ischemic patient in the treatment of MI using a fully grown non-immune-compromised rat model. Mesenchymal stem cells were isolated from adipose tissue and bone marrow and compared with respect to surface markers and proliferative capability. To compare the regenerative potential of the two stem cell populations, male Sprague–Dawley rats were randomized to receive intramyocardial injections of adipose-derived stem cells, bone marrow-derived mesenchymal stem cells, or phosphate-buffered saline 1 week following induction of MI. After 4 weeks, left ventricular ejection fraction (LVEF) was improved in the adipose-derived stem cell group, and scar wall thickness was greater compared with the saline group. Adipose-derived as well as bone marrow-derived mesenchymal stem cells prevented left ventricular end diastolic dilation. Neither of the cell groups displayed increased angiogenesis in the myocardium compared with the saline group. Adipose-derived stem cells from a human ischemic patient preserved cardiac function following MI, whereas this could not be demonstrated for bone marrow-derived mesenchymal stem cells, with only adipose-derived stem cells leading to an improvement in LVEF. Neither of the stem cell types induced myocardial angiogenesis, raising the question whether donor age and health have an effect on the efficacy of stem cells used in the treatment of MI.

【 授权许可】

Unknown   
© 2014 Cognizant Comm. Corp.

【 图 表 】

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