Cell Viability and Noninvasive In Vivo MRI Tracking of 3D Cell Encapsulating Self-Assembled Microcontainers

Cell Transplantation

Article

David H. Gracias¹  Timothy Leong¹  Barjor Gimi²  Zaver M. Bhujwalla³  Matthias Stuber³  Dmitri Artemov³  Wesley Gilson³ 

[1] Department of Chemical Engineering, Johns Hopkins University, Baltimore, MD, USA;Department of Radiology, University of Texas Southwestern Medical Center at Dallas, Dallas, TX, USA; The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA;The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD, USA;

关键词: Cell therapy;    Cell encapsulation;    Magnetic resonance microscopy;    Self-assembly;    Microcapsules;    Implantation;   

DOI  :  10.3727/000000007783464803

 received in 2006-08-24, accepted in 2006-10-20,  发布年份 2007

来源: Sage Journals

【 摘 要 】

Several molecular therapies require the implantation of cells that secrete biotherapeutic molecules and imaging the location and microenvironment of the cellular implant to ascertain its function. We demonstrate noninvasive in vivo magnetic resonance imaging (MRI) of self-assembled microcontainers that are capable of cell encapsulation. Negative contrast was obtained to discern the microcontainer with MRI; positive contrast was obtained in the complete absence of background signal. MRI on a clinical scanner highlights the translational nature of this research. The microcontainers were loaded with cells that were dispersed in an extracellular matrix, and implanted both subcutaneously and in human tumor xenografts in SCID mice. MRI was performed on the implants, and microcontainers retrieved postimplantation showed cell viability both within and proximal to the implant. The microcontainers are characterized by their small size, three dimensionality, controlled porosity, ease of parallel fabrication, chemical and mechanical stability, and noninvasive traceability in vivo.

【 授权许可】

Unknown   
© 2007 Cognizant Comm. Corp.