期刊论文

【摘要】

Despite the success of alginate scaffolds and mesenchymal stem cells (MSCs) therapy in cardiac failure treatment, the impact of the physicochemical environment provided by alginate matrices on cell behavior has never been investigated. The purpose of this work was double: to determine the alginate composition influence on (1) encapsulated rat MSC viability, paracrine activity, and phenotype in vitro and (2) cardiac implantability and in vivo biocompatibility of patch shape scaffolds. Two alginates, differing in composition and thus presenting different mechanical properties when hydrogels, were characterized. In both cases, encapsulated MSC viability was maintained at around 75%, and their secretion characteristics were retained 28 days postencapsulation. In vivo study revealed a high cardiac compatibility of the tested alginates: cardiac parameters were maintained, and rats did not present any sign of infection. Moreover, explanted hydrogels appeared surrounded by a vascularized tissue. However, scaffold implantability was highly dependent on alginate composition. G-type alginate patches, presenting higher elastic and Young moduli than M-type alginate patches, showed a better implantation easiness and were the only ones that maintained their shape and morphology in vivo. As a consequence of alginate chemical composition and resulting hydrogel structuration, G-type alginate hydrogels appear to be more adapted for cardiac implantation.

【授权许可】

Unknown
© 2012 Cognizant Comm. Corp.

【预览】

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RO202212200392587ZK.pdf	687KB	PDF	download
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Cell Transplantation
Alginate Scaffolds for Mesenchymal Stem Cell Cardiac Therapy: Influence of Alginate Composition
Article
Olivier Lairez¹ Chiara Alfarano¹ Denis Calise² Daniel Cussac² Angelo Parini³ Brigitte Sallerin³ Sophie Girod Fullana⁴ Caroline Ceccaldi⁵
[1] INSERM, U858, Toulouse, France;INSERM, U858, Toulouse, France;Université de Toulouse, UPS, Faculté des Sciences Pharmaceutiques, Toulouse, France;INSERM, U858, Toulouse, France;Université de Toulouse, UPS, Faculté des Sciences Pharmaceutiques, Toulouse, France;CHU Toulouse, Service de Pharmacie Hospitalière, Toulouse, France;Université de Toulouse, CIRIMAT, UPS-INPT-CNRS, Faculté de Pharmacie, Toulouse, France;Université de Toulouse, CIRIMAT, UPS-INPT-CNRS, Faculté de Pharmacie, Toulouse, France;INSERM, U858, Toulouse, France;
关键词: Alginate; Cell encapsulation; Mesenchymal stem cells; Biomimetic materials; Heart;
DOI : 10.3727/096368912X647252
received in 2010-10-13, accepted in 2011-10-28, 发布年份 2012
来源: Sage Journals
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【 摘 要 】

【 授权许可】

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【 图 表 】

【 参考文献 】

【摘要】

【授权许可】

【预览】

【图表】

【参考文献】