Prognostic value of metabolic signature on 18F-FDG uptake in breast cancer patients after radiotherapy

Molecular Therapy: Oncolytics

Xin Mei¹  Zhaozhi Yang¹  Emmanuel Deshayes¹  Jiong Wu¹  Xiaomeng Zhang²  Li Zhang³  Zhimin Shao³  Xiaomao Guo³  Jinli Ma³  Jin Meng⁴  Xingxing Chen⁴  Yizhou Jiang⁴  Xiaoli Yu⁴  Wei Shi⁵ 

[1] Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China;Nuclear Medicine Department, Institut régional du Cancer de Montpellier (ICM), University of Montpellier, 34298 Montpellier Cedex 5, France;Shanghai Key Laboratory of Radiation Oncology, Shanghai 200032, China;Department of Radiation Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, China;Institut de Recherche en Cancérologie de Montpellier (IRCM), INSERM U1194, Institut régional du Cancer de Montpellier (ICM), University of Montpellier, 34298 Montpellier Cedex 5, France;

关键词: breast cancer;    radiotherapy;    FDG uptake;    gene signature;    immunosuppression;   

DOI  :  

来源: DOAJ

【 摘 要 】

Radiotherapy (RT) is a major modality of postoperative treatment in breast cancer. The maximal standardized value (SUVmax) is 18FDG-PET/CT derived parameter that reported to be a valuable prognostic factor in cancer patients. Herein, we aimed to identify a prognostic gene signature associated with glucose uptake for breast cancer patients after RT by leveraging the mRNA expression profiling on public datasets. The glucose uptake signature was constructed using the single sample gene set enrichment analysis (ssGSEA) algorithm and evaluated in GSE21217 where SUVmax value was measured by PET-CT directly. The prognostic value was validated in three post-RT breast cancer cohorts (GSE103744, NKI, and FUSCC databases). The patients were stratified into glucose uptake signature score-high and low groups. Patients with a higher score had worse survival than those with a lower score. Mechanistically, the glucose uptake signature was calculated in each cell type of a single-cell RNA-seq database from five breast cancer patients. Glucose uptake signature score was significantly elevated in the malignant epithelial cells compared with normal ones. The immunosuppression markers including PDCD1, TIGIT, LAG3, and HAVCR2 were significantly upregulated in the T cells bearing a high glucose uptake signature score. Collectively, our results demonstrated the potential prognostic value of a glucose uptake signature in the post-RT breast cancer patients.

【 授权许可】

Unknown