Anti-Oxidative and Anti-Apoptotic Effects of Apigenin on Number of Viable and Apoptotic Blastomeres, Zona Pellucida Thickness and Hatching Rate of Mouse Embryos

International Journal of Fertility and Sterility

Houman Parsaie¹  Hamid Reza Sameni¹  Manouchehr Safari¹  Mohammad Reza Aldaghi¹  Sam Zarbakhsh¹ 

[1] Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran;

关键词: apigenin;    apoptosis;    blastomeres;    embryonic development;    zona pellucida;   

DOI  :  10.22074/ijfs.2018.5392

来源: DOAJ

【 摘 要 】

BackgroundApigenin is a plant-derived compound belonging to the flavonoids category and bears protective effects on different cells. The aim of this study was to evaluate the effect of apigenin on the number of viable andapoptotic blastomeres, the zona pellucida (ZP) thickness and hatching rate of pre-implantation mouse embryos exposedto H2O2 and actinomycin D.Materials and MethodsIn this experimental study, 420 two-cell embryos were randomly divided into six groups:i. Control, ii. Apigenin, iii. H2O2 , iv. Apigenin+H2O2 , v. Actinomycin D, and vi. Apigenin+Actinomycin D. The percentage of blastocysts and hatched blastocysts was calculated. Blastocyst ZP thickness was also measured. In addition, viable blastomeres quantity was counted by Hoechst and propidium iodide staining and the number of apoptoticblastomeres was counted by TUNEL assay.ResultsThe results of viable and apoptotic blastomeres quantity, the ZP thickness, and the percentage of blastocysts and hatched blastocysts were significantlymore favorable in the apigenin group, rather than the controlgroup (P < 0.05). The results of the apigenin+H2O2 group were significantly more favorable than the H2O2 group(P < 0.05); and the results of apigenin+actinomycin D group were significantly more favorable than actinomycin Dgroup (P < 0.05).ConclusionThe results suggest that apigenin may protect mouse embryos against H2O2 and actinomycin D. So thatit increases the number of viable blastomeres and decreases the number of apoptotic blastomeres, which may causeexpanding the blastocysts, thinning of the ZP thickness and increasing the rate of hatching in mouse embryos.

【 授权许可】

Unknown