Protection against Cyclosporine-Induced Reprotoxicity by Satureja khuzestanica Essential Oil in Male Rats

International Journal of Fertility and Sterility

Zahra Akbarizadeh¹  Farah Farokhi¹  Ali Shalizar Jalali²  Gholamreza Najafi² 

[1] Department of Biology, Faculty of Science, Urmia University, Urmia, Iran;Histology and Embryology Research Laboratories, Department of Basic Sciences, Faculty of Veterinary Medicine,Urmia University, Urmia, Iran;

关键词: cyclosporine;    sperm;    in vitro fertilization;   

DOI  :  10.22074/ijfs.2016.4615

来源: DOAJ

【 摘 要 】

Background The effects of cyclosporine (Cs), a fungal cyclic polypeptide with potent immunosuppressive activity, on fertility have assumed greater significance with the increasing numbers of transplantations being performed all over the world. Current study was undertaken to investigate the potential of Satureja khuzestanica Essential Oil (SEO) as an antioxidant to mitigate Cs-induced reprotoxicity. Materials and Methods In this experimental study (April-July 2012), thirty-two adult male Wistar rats were randomly divided into 4 groups of 8 animals each. Two groups of rats were administered Cs [40 mg/kg/day, per oral (p.o.)] for 45 days. One of these groups received SEO (225 mg/kg/day, p.o.) four hours after Cs administration. A vehicle-treated control group and a SEO control group were also included. Epididymal sperm characteristics, in vitro fertilizing capacity as well as embryo development were evaluated. For statistical analysis, one-way ANOVA and Tukey’s post-hoc test were used, and the value of P < 0.05 was considered as the criterion for statistical significance. Results Sperm count and viability along with fertilization and blastocyst development rates were significantly decreased by Cs treatment. Moreover, Cs-treated group showed significant increases in DNA damage, protamine deficiency of the sperm cells and proportion of spermatozoa with cytoplasmic droplet. Notably, aforementioned parameters were improved to near normal level by SEO co-administration. Conclusion These results suggest that SEO has a protective action against Cs-induced reprotoxicity in a rat model.

【 授权许可】

Unknown