Comparative study of fertilization rates of C57BL/6NKorl and C57BL/6N mice obtained from two other sources

Laboratory Animal Research

Kil Soo Kim¹  Hye Ryeong Kim²  Dae Youn Hwang²  Mi Rim Lee²  Woo Bin Yun²  Jun Young Choi²  Jin Ju Park²  Bo Ram Song²  Ji Eun Kim²  Joon Young Cho³  Young Suk Jung⁴  Hyun Keun Song⁵ 

[1] College of Veterinary Medicine, Kyungpook National University;Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University;Department of Health and Exercise Science, Korea National Sport University;Department of Pharmacy, College of Pharmacy, Pusan National University;Departmentof Microbiology and Immunology, Inje University College of Medicine;

关键词: C57BL/6NKorl;    reproductive ability;    sperm;    egg;    in vitro fertilization;    embryo development rate;   

DOI  :  10.5625/lar.2017.33.2.179

来源: DOAJ

【 摘 要 】

Abstract C57BL/6N is the most widely used inbred mouse strain applied in a wide variety of research areas including cancer, cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research. To compare the fertilization rates of C57BL/6NKorl mice with two commercial C57BL/6N stocks, differences in reproductive organ structures, sperm and egg numbers, fertilization rates, and embryo development rates among C57BL/6NKorl (Korea FDA source), C57BL/6NA (USA source), and C57BL/6NB (Japan source) mice were determined. Among the stocks, no significant differences were detected in organ weight and histological structure of male and female reproductive organs, although body weight was higher in C57BL/6NKorl mice than that in the other groups. The concentration and morphology of sperm and eggs in C57BL/6NKorl mice were similar to those of C57BL/6NA and C57BL/6NB mice. Furthermore, the three stocks had similar in vitro fertilization and embryo development rates, although these rates tended to be higher in C57BL/6NB mice. Pup body weight was higher in C57BL/6NKorl and C57BL/6NB mice than that in C57BL/6NA mice. The results of the present study suggest that C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice obtained from three different sources have similar fertilization and embryo development rates, although there were slight differences in the magnitude of their responses rates.

【 授权许可】

Unknown