期刊论文详细信息
BMC Genomics
A new reference genome for Sorghum bicolor reveals high levels of sequence similarity between sweet and grain genotypes: implications for the genetics of sugar metabolism
Zachary W. Brenton1  Barry S. Flinn1  Elizabeth A. Cooper1  Stephen Kresovich1  Kerrie Barry2  Anna Lipzen2  Chris Daum2  Yuko Yoshinaga2  Shengqiang Shu2  Penny Xia3  Christopher Saski3  Wilfred Vermerris4  Dave Flowers5  Jerry Jenkins5  Jeremy Schmutz5  Feng Luo6  Yunsheng Wang6 
[1] Advanced Plant Technology Program, Clemson University;Department of Energy, Joint Genome Institute;Department of Genetics and Biochemistry, Clemson University;Department of Microbiology and Cell Science and UF Genetics Institute, University of Florida;HudsonAlpha Institute for Biotechnology;School of Computing, Clemson University;
关键词: Sorghum;    Sugar metabolism;    Sugar transport;    Genomics;    Gene expression;   
DOI  :  10.1186/s12864-019-5734-x
来源: DOAJ
【 摘 要 】

Abstract Background The process of crop domestication often consists of two stages: initial domestication, where the wild species is first cultivated by humans, followed by diversification, when the domesticated species are subsequently adapted to more environments and specialized uses. Selective pressure to increase sugar accumulation in certain varieties of the cereal crop Sorghum bicolor is an excellent example of the latter; this has resulted in pronounced phenotypic divergence between sweet and grain-type sorghums, but the genetic mechanisms underlying these differences remain poorly understood. Results Here we present a new reference genome based on an archetypal sweet sorghum line and compare it to the current grain sorghum reference, revealing a high rate of nonsynonymous and potential loss of function mutations, but few changes in gene content or overall genome structure. We also use comparative transcriptomics to highlight changes in gene expression correlated with high stalk sugar content and show that changes in the activity and possibly localization of transporters, along with the timing of sugar metabolism play a critical role in the sweet phenotype. Conclusions The high level of genomic similarity between sweet and grain sorghum reflects their historical relatedness, rather than their current phenotypic differences, but we find key changes in signaling molecules and transcriptional regulators that represent new candidates for understanding and improving sugar metabolism in this important crop.

【 授权许可】

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