Safety and Effectiveness of Copaiba Oleoresin (C. reticulata Ducke) on Inflammation and Tissue Repair of Oral Wounds in Rats

International Journal of Molecular Sciences

CarlosAugusto Galvão Barboza¹  JuliaTurra Ribeiro²  ManoelaDomingues Martins²  OsmarAlves Lameira³  MaríaOlimpia Paz Alvarenga⁴  LeonardoOliveira Bittencourt⁴  PauloFernando Santos Mendes⁴  RafaelRodrigues Lima⁴  MartaChagas Monteiro⁵ 

[1] Department of Morphology, Federal University of Rio Grande do Norte, Natal 59078-900, Brazil;Faculty of Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90040-060, Brazil;Laboratory of Biotechnology, Embrapa Amazônia Oriental, Belém 66075-110, Brazil;Laboratory of Functional and Structural Biology, Biological Sciences Institute, Federal University of Pará, Belém 66075-110, Brazil;Laboratory of In Vitro Tests, Immunology and Microbiology, Health Science Institute, Federal University of Pará, Belém 66075-110, Brazil;

关键词: copaiba oleoresin;    wound healing;    alternative therapy;    natural anti-inflammatory;    C. reticulata Ducke;   

DOI  :  10.3390/ijms21103568

来源: DOAJ

【 摘 要 】

In traditional communities of the Brazilian Amazon, the copaiba oleoresin (C. reticulata Ducke) is widely known for its therapeutic activity, especially its wound healing and anti-inflammatory actions. Our study aimed to evaluate these effects in oral lesions and the safety of the dosage proposed. A punch biopsy wound was induced on the ventral surface of the tongue of forty-five male Wistar rats under anesthesia. Animals were randomly allocated to one of three groups based on the treatment: control, corticoid and copaiba. A daily dose of each treatment and vehicle was administrated by oral gavage for three consecutive days. Sample collections took place on the third, seventh and 15th days post-wounding for clinical and histopathological analyses. Blood was collected on the third and seventh days for kidneys and liver function tests. Semi-quantitative analyses were performed based on scores of inflammation and reepithelization. Tissue collagen deposition was detected by PicroSirius red staining. Copaiba-treated wounds revealed a smaller wound area, decreased of acute inflammatory reaction and enhanced reepithelization. The levels of kidney and liver function tests did not reveal presence of damage post-treatments. Our findings suggest that copaiba oleoresin is a safe and effective alternative therapy for inflammation and tissue repair of oral wounds in this animal model.

【 授权许可】

Unknown