Antimicrobial Evaluation of Diterpenes from Copaifera langsdorffii Oleoresin Against Periodontal Anaerobic Bacteria

Molecules

Ariana B. Souza¹  Maria G. M. de Souza¹  Maísa A. Moreira¹  Monique R. Moreira¹  Niege A. J. C. Furtado¹  Carlos H. G. Martins¹  Jairo K. Bastos¹  Raquel A. dos Santos¹  Vladimir C. G. Heleno¹  Sergio Ricardo Ambrosio¹ 

[1] ¹Nucleus of Research in Sciences and Technology, University of Franca, Franca, SP, 14404-600, Brazil ²Faculty of Pharmaceutical Sciences of Ribeirao Preto, University of Sao Paulo, Ribeirao Preto, SP, 14040-903, Brazil

关键词: copalic acid;    periodontitis;    Porphyromonas gingivalis;    copaiba oleoresin;    Copaifera langsdorffii;   

DOI  :  10.3390/molecules16119611

来源: mdpi

【 摘 要 】

The antimicrobial activity of four labdane-type diterpenes isolated from the oleoresin of Copaifera langsdorffii as well as of two commercially available diterpenes (sclareol and manool) was investigated against a representative panel of microorganisms responsible for periodontitis. Among all the evaluated compounds, (−)-copalic acid (CA) was the most active, displaying a very promising MIC value (3.1 µg mL⁻¹; 10.2 µM) against the key pathogen (Porphyromonas gingivalis) involved in this infectious disease. Moreover, CA did not exhibit cytotoxicity when tested in human fibroblasts. Time-kill curve assays performed with CA against P. gingivalis revealed that this compound only inhibited the growth of the inoculums in the first 12 h (bacteriostatic effect). However, its bactericidal effect was clearly noted thereafter (between 12 and 24 h). It was also possible to verify an additive effect when CA and chlorhexidine dihydrochloride (CHD, positive control) were associated at their MBC values. The time curve profile resulting from this combination showed that this association needed only six hours for the bactericidal effect to be noted. In summary, CA has shown to be an important metabolite for the control of periodontal diseases. Moreover, the use of standardized extracts based on copaiba oleoresin with high CA contents can be an important strategy in the development of novel oral care products.

【 授权许可】

CC BY   
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.