Dihydroartemisinin inhibits TCTP-dependent metastasis in gallbladder cancer

Journal of Experimental & Clinical Cancer Research

Qiang Ma¹  Zheng Wang¹  Xuefeng Wang¹  Haibin Liang¹  Wei Gong¹  Shanshan Xiang¹  Yong Zhang¹  Fei Zhang¹  Yijian Zhang¹  Huaifeng Li¹  Lin Jiang¹  Yingbin Liu¹  Yunping Hu¹  Yuanyuan Ye¹  Zihang Xu² 

[1] Department of General Surgery, Xinhua Hospital affiliated to Shanghai Jiao Tong University School of Medicine;Laboratory of Integrative Medicine, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine;

关键词: Metastasis;    Invasion;    Gallbladder cancer;    TCTP;    Dihydroartemisinin;   

DOI  :  10.1186/s13046-017-0531-3

来源: DOAJ

【 摘 要 】

Abstract Background Patients with metastatic or relapsed gallbladder cancer generally have a poor prognosis. Therefore, targeting metastasis is one arm of therapeutic strategies to treat gallbladder cancer. Methods Levels of translationally controlled tumor protein (TCTP) were measured in samples of gallbladder cancer by immunohistochemical staining. Wound healing, migration and invasion assays were used to investigate the motility of cells. Western blot assay was used to investigate the levels of TCTP and other proteins. Liver metastasis models and lung metastasis models were established to investigate the inhibitory effect of Dihydroartemisinin on gallbladder cancer metastasis. Results TCTP is aberrantly expressed in gallbladder cancer patients and associated with metastasis and a poor prognosis. Depleting TCTP significantly inhibited gallbladder cancer cell migration and invasion. We found that Dihydroartemisinin as a potent inhibitor of TCTP inhibited TCTP-dependent cell migration and invasion by reducing cell division control protein 42 homolog (Cdc42) activation. In addition, in mice with xenografted tumors, treatment with Dihydroartemisinin decreased gallbladder cancer cell metastases and improved survival. Conclusions These findings provide new insights into the therapeutic activity of Dihydroartemisinin as a treatment for gallbladder cancer metastasis.

【 授权许可】

Unknown