Propofol induces proliferation and invasion of gallbladder cancer cells through activation of Nrf2

Journal of Experimental & Clinical Cancer Research

Mingxin Zhang¹  Guixia Jing⁴  Wenguang Ye¹  Suna Zhou²  Ning Wang³  Lingmin Zhang⁴ 

[1] Department of Gastroenterology, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shaanxi Province, 710038, China;Department of Thoracic Oncosurgery, First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi’an, Shaanxi Province, 710061, China;Department of Anesthesiology, Second Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi’an, Shaanxi Province, 710061, China;Department of Anesthesiology, First Affiliated Hospital, Medical School, Xi'an Jiaotong University, Xi’an, Shaanxi Province, 710061, China

关键词: Invasion;    Proliferation;    Gallbladder cancer;    NF-E2-related factor 2 (Nrf2);    Propofol;   

Others  :  826162
DOI  :  10.1186/1756-9966-31-66

 received in 2012-06-24, accepted in 2012-08-14,  发布年份 2012

【 摘 要 】

Background

Propofol is one of the most commonly used intravenous anaesthetic agents during cancer resection surgery, but the effect of propofol on gallbladder cancer is not clear. NF-E2-related factor 2 (Nrf2) is abundantly expressed in cancer cells and relates to proliferation, invasion, and chemoresistance. The aims of the current study were to evaluate effects of propofol on the behavior of human GC cells and role of Nrf2 in these effects.

Method

The effects of propofol on cell proliferation, apoptosis, and invasion were detected by MTT assays, flow cytometry, and transwell assay. Also, activation of Nrf2 was determined by western blot, RT-PCR, and immunofluorescence assays. Nrf2 was knocked-down in GBC-SD cells by shRNA before evaluating the role of Nrf2 in the influence of propofol on biological behaviors.

Results

Propofol promoted the proliferation of GBC-SD cells in a dose- and time- dependent manner. After exposure to propofol for 48 h, GBC-SD cells showed decreased apoptosis and increased invasion. Also, propofol over-expressed Nrf2 at both the protein and mRNA levels and induced translocation of Nrf2 into the nucleus. Finally, loss of Nrf2 by shRNA reversed the effect of propofol on cell proliferation, apoptosis, and invasion.

Conclusion

Propofol induces proliferation and promotes invasion of GC cells through activation of Nrf2.

【 授权许可】

   
2012 Zhang et al.; licensee BioMed Central Ltd.

【 图 表 】

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