Genetic Diversity Analysis of CIMMYT Bread Wheat (Triticum aestivum L.) Lines by SRAP Markers

Electronic Journal of Plant Breeding

关键词: Bread wheat;    CIMMYT;    genetic diversity;    SRAP;    Triticum aestivum;   

DOI  :  

来源: DOAJ

【 摘 要 】

Genetic diversity is one of the key factors for the improvement of many crop plants including wheat. Many wheat scientistshave studied genetic diversity in wheat germplasm using different molecular markers which have provided a powerfulapproach to analyze genetic relationships among wheat germplasms. In this study, genetic diversity of CIMMYT(International maize and wheat improvement center) bread wheat lines collected from Russia was evaluated using 30sequence-related amplified polymorphism (SRAP) primer combinations. 686 DNA band was obtained from the 23 primercombinations and approximately 90% of them were found to be polymorphic. Ratio of polymorphic loci, Shannon'sdiversity index and gene diversity were found 82.61%, 0.39 and 0.26 respectively. The three main clusters were found byusing UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis method and the average rate ofgenetic similarity with 0.462. Two main clusters were shown in principal component analysis (PCA) which is consistentwith the result of UPGMA. It can be concluded that SRAP markers can be used for wheat genetic diversity studies and havepotential linkage mapping, molecular characterizations and marker assisted selection (MAS) breeding.

【 授权许可】

Unknown