Grade C molar-incisor pattern periodontitis subgingival microbial profile before and after treatment

Journal of Oral Microbiology

Jennifer Barb¹  Hong Huang²  Peter Harrison²  Ikramuddin Aukhil²  Luciana Shaddox²  Irina M. Velsko³  Natalia Chalmers⁴ 

[1] Clinical Center, National Institutes of Health;College of Dentistry, University of Florida;Max Planck Institute for the Science of Human History;NIH Clinical Center;

关键词: aggressive periodontitis;    subgingival microbiota;    a.a.;    therapy;    microarray;   

DOI  :  10.1080/20002297.2020.1814674

来源: DOAJ

【 摘 要 】

Aim: This study evaluated the influence of periodontal therapy on the microbiological profile of individuals with Grade C Molar-Incisor Pattern Periodontitis (C/MIP). Methods: Fifty-three African-American participants between the ages of 5–25, diagnosed with C/MIP were included. Patients underwent full mouth mechanical debridement with systemic antibiotics (metronidazole 250 mg + amoxicillin 500 mg, tid, 7 days). Subgingival samples were collected from a diseased and a healthy site from each individual prior to treatment and at 3, 6, 12, 18 and 24 months after therapy from the same sites. Samples were subjected to a 16S rRNA gene based-microarray. Results: Treatment was effective in reducing the main clinical parameters of disease. Aggregatibacter actinomycetemcomitans (A.a.) was the strongest species associated with diseased sites. Other species associated with diseased sites were Treponema lecithinolyticum and Tannerella forsythia. Species associated with healthy sites were Rothia dentocariosa/mucilaginosa, Eubacterium yurii, Parvimonas micra, Veillonella spp., Selenomonas spp., and Streptococcus spp. Overall, treatment was effective in strongly reducing A.a. and other key pathogens, as well as increasing health-associated species. These changes were maintained for at least 6 months. Conclusions:Treatment reduced putative disease-associated species, particularly A.a., and shifted the microbial profile to more closely resemble a healthy-site profile. (Clinicaltrials.gov registration #NCT01330719).

【 授权许可】

Unknown