Subgingival Microbiota of Mexicans with Type 2 Diabetes with Different Periodontal and Metabolic Conditions

International Journal of Environmental Research and Public Health

卷:16

Laurie-Ann Ximenez-Fyvie¹  Adriana-Patricia Rodríguez-Hernández¹  Maríade Lourdes Márquez-Corona²  CarloEduardo Medina-Solís²  AméricaPatricia Pontigo-Loyola² 

[1] Laboratory of Molecular Genetics, School of Dentistry, National Autonomous University of Mexico (UNAM), Mexico City 04360, Mexico;

[2] The Academic Area of Dentistry in the Health Sciences Institute, the Autonomous University of the State of Hidalgo, Pachuca 42039, Mexico;

关键词: Type 2 diabetes mellitus;    periodontitis;    subgingival microbiota;    “Checkerboard” DNA–DNA hybridization;    obesity;    glycemic poor control;    dyslipidemia;   

DOI  :  10.3390/ijerph16173184

来源: DOAJ

【 摘 要 】

Background: Type-2-Diabetes (T2D) and Periodontitis are major inflammatory diseases. However, not much is known about the specific subgingival microbiota in Mexicans with diabetes and metabolic dysbiosis. The aim of this study was to describe the subgingival microbiota of Mexicans with T2D and the different periodontal and metabolic conditions, through “Checkerboard” DNA−DNA hybridization. Methods: Subjects were divided into two groups—periodontal-health (PH) (PH_non-T2D; n = 59, PH_T2D; n = 14) and generalized-periodontitis (GP) (GP_non-T2D; n = 67, GP_T2D; n = 38). Obesity (BMI ≥ 30 kg/m²) and serum levels of glycated-hemoglobin (HbA1c), total-lipids, triglycerides, total-cholesterol, high-density-lipids, and low-density-lipids were measured for the T2D individuals. Subgingival microbial identification was processed for 40 species through DNA-probes. Results: Subjects with T2D harbored significantly higher mean total levels (PH: p < 0.001, and GP_NS), a lower proportion of “red” complex (GP: p < 0.01), a higher proportion of “yellow” (GP; p < 0.001), and “orange” (GP; p < 0.01) complex than the non-T2D. GP_T2D individuals exhibited a greater proportion of putative-species—Campylobacter gracilis and S. constellatus (p < 0.001), and Parvimonas micra and Prevotella nigrescens (p < 0.01), than GP_non-T2D. T2D individuals with HbA1c > 8% had presented significantly higher mean pocket-depth and higher levels of G. morbillorum (p < 0.05) and those with obesity or dyslipidemia harbored higher levels, prevalence, or proportion of Streptococcus sp., Actinomyces sp., and Capnocytophaga sp. Conclusions: T2D individuals harbored a particular microbial profile different to non-T2D microbiota. Metabolic control was related to dysbiosis of microbiota—HbA1c>8% related to periodontitis and obesity or dyslipidemia with the predominance of saccharolytic bacteria, irrespective of their periodontal condition.

【 授权许可】

Unknown