Dendropanax trifidus Sap-Mediated Suppression of Obese Mouse Body Weight and the Metabolic Changes Related with Estrogen Receptor Alpha and AMPK-ACC Pathways in Muscle Cells

Nutrients

Ik-Hyun Cho¹  Young Joo Lee²  Namkyu Lee²  Dalnim Kim³  Ahreum Lee³  Hyun-Jeong Yang³  Soo Min Cho⁴  Eugene Koh⁵ 

[1] College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea;Department of Integrated Bioscience and Biotechnology, College of Life Science, Sejong University, Seoul 05006, Korea;Korea Institute of Brain Science, Seoul 06022, Korea;PharmCADD, R&D Center, Seoul 06180, Korea;Temasek Life Sciences Laboratories, Singapore 117604, Singapore;

关键词: Dendropanax trifidus;    body weight;    C2C12;    metabolism;    glycolysis;    mitochondrial respiration;   

DOI  :  10.3390/nu14051098

来源: DOAJ

【 摘 要 】

Dendropanax trifidus (DT) is a medicinal herb native to East Asia, which has been used extensively for its therapeutic properties in traditional medicine. In this study, we examined the effects of DT sap on the regulation of body weight and muscle metabolism in mice. Obese model db/db mice were administered daily with DT sap or vehicle control over a 6-week period. The effects of DT sap on muscle metabolism were studied in C2C12 muscle cells, where glycolytic and mitochondrial respiration rates were monitored. As AMP-activated protein kinase (AMPK) is a master regulator of metabolism and plays an important function as an energy sensor in muscle tissue, signaling pathways related with AMPK were also examined. We found that DT sap inhibited body weight increase in db/db, db/+, and +/+ mice over a 6-week period, while DT sap-treated muscle cells showed increased muscle metabolism and also increased phosphorylation of AMPK and Acetyl-CoA Carboxylase (ACC). Finally, we found that DT sap, which is enriched in estrogen in our previous study, significantly activates estrogen alpha receptor in a concentration-dependent manner, which can drive the activation of AMPK signaling and may be related to the muscle metabolism and weight changes observed here.

【 授权许可】

Unknown