Intestinal and Mucosal Microbiome Response to Oral Challenge of Enterotoxigenic Escherichia coli in Weaned Pigs

Pathogens

Lianqiang Che¹  Qiuhong Chen¹  Ying He¹  Yingjie Li¹  Shan-Shan Peng²  Ping-Ping Jiang²  Qi Hu³ 

[1] Key Laboratory for Animal Disease Resistance and Nutrition of the Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China;School of Public Health, Sun Yat-sen University, Guangzhou 510080, China;The Neomics Institute, Shenzhen 518122, China;

关键词: infant diarrhoea;    enterotoxigenic Escherichia coli;    proteomics;    mucosal microbiome;    regularised canonical correlation analysis;    bile acid;   

DOI  :  10.3390/pathogens11020160

来源: DOAJ

【 摘 要 】

Enterotoxigenic Escherichia coli (ETEC) is closely associated with diarrhoea in children in resource-limited countries. This study aims to investigate the change of the mucosal microbiome and protein expression in the ileum induced by E. coli K88 (ETEC) using pigs as a model. Seven weaned male pigs were orally given ETEC (1 × 10⁹ CFU, n = 7), and the other seven received saline (CON, n = 7). Ileal tissues were obtained 48 hours after the ETEC challenge for both proteomic and mucosal microbiome analyses. Nine proteins were found with altered abundance between the two groups, including a decrease in FABP1 and FABP6, involved in bile acid circulation. The TLR-9 mediated pathway was also affected showing increased transcription of genes SIGIRR and MyD88. Correlations between the ileal proteins and mucosal bacterial taxa found included a positive correlation between Lactobacilllus and PPP3CA (r = 0.9, p < 0.001) and a negative correlation between Prevotella with CTNND1 (r = −0.7, p < 0.01). In conclusion, ETEC infection caused inflammation and impaired the circulation of bile acids and the mucosal microbiome may affect the expression of intestinal proteins. Further studies are needed to explain the exact roles of these affected processes in the pathogenesis of ETEC-triggered diarrhoea.

【 授权许可】

Unknown