Preventive Effect of Depolymerized Sulfated Galactans from Eucheuma serra on Enterotoxigenic Escherichia coli-Caused Diarrhea via Modulating Intestinal Flora in Mice

Marine Drugs

Chao Zou¹  Yu Ma¹  Wenqiang Liu¹  Zhaohua Chen¹  Qian Zhang¹  Yixiang Liu¹  Yanbo Wang¹  Linglin Fu² 

[1] College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310018, China;

关键词: Eucheuma serra;    sulfated polysaccharide;    enterotoxigenic Escherichia coli;    bacterial diarrhea;    16S rRNA;    gut microbiota;   

DOI  :  10.3390/md19020080

来源: DOAJ

【 摘 要 】

In this work, the preventive effect of depolymerized sulfated polysaccharides from Eucheuma serra (DESP) on bacterial diarrhea by regulating intestinal flora was investigated in vivo. Based on the enterotoxigenic Escherichia coli (ETEC)-infected mouse diarrhea model, DESP at doses ranging from 50 mg/kg to 200 mg/kg alleviated weight loss and decreased the diarrhea rate and diarrhea index. Serological tests showed that the levels of inflammation-related factors were effectively suppressed. Furthermore, the repaired intestinal mucosa was verified by morphology and pathological tissue section observations. Compared with the model group, the richness and diversity of the intestinal flora in the DESP group increased according to the 16S rRNA high-throughput sequencing of the gut microbiota. Specifically, Firmicutes and Actinobacteria increased, and Proteobacteria decreased after DESP administration. At the family level, DESP effectively improved the abundance of Lactobacillaceae, Bifidobacteriaceae, and Lachnospiraceae, while significantly inhibiting the growth of Enterobacteriaceae. Therefore, the antimicrobial diarrhea function of DESP may be related to the regulation of intestinal microbiota.

【 授权许可】

Unknown