Molecular Phylogenetics and Biological Potential of Fungal Endophytes From Plants of the Sundarbans Mangrove

Frontiers in Microbiology

Sabiha Sultana¹  Md Afjalus Siraj²  Veronique Seidel³  Md Amirul Islam⁴  Md Sohanur Rahaman⁴ 

[1] Agrotechnology Discipline, Life Science School, Khulna University, Khulna, Bangladesh;Department of Pharmaceutical Sciences, Daniel K. Inouye College of Pharmacy, University of Hawaii at Hilo, Hilo, HI, United States;Natural Products Research Laboratory, Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, United Kingdom;Pharmacy Discipline, Life Science School, Khulna University, Khulna, Bangladesh;

关键词: bioactivity;    ecosystem;    endophytic fungi;    phylogenetics;    fungal metabolites;    symbiosis;   

DOI  :  10.3389/fmicb.2020.570855

来源: DOAJ

【 摘 要 】

The Sundarbans forest in Bangladesh is the world’s largest mangrove. It is a unique ecosystem where living organisms face extreme challenges to compete for survival. Such competition results in the production of bioactive molecules which are useful for agriculture and human health. In this study, eighty fungal endophytes from nine mangrove plants growing in a region, as yet unexplored, of the Sundarbans were isolated by surface sterilisation and pure culture techniques. Among the eighty isolates subjected to a preliminary antimicrobial screening using an agar plug diffusion assay, only fifteen showed some promising activity. These were subsequently identified by polymerase chain reaction of their ITS gene. Extracts prepared from the identified isolates were screened for antimicrobial, antioxidant, cytotoxic and α-glucosidase inhibitory activities. Their total polyphenol and flavonoid content and their FRAP value were also determined. All endophytes are reported for the first time in the plants under investigation.

【 授权许可】

Unknown