The Newly Isolated Endophytic Fungus Paraconiothyrium sp. LK1 Produces Ascotoxin

Molecules

Abdul Latif Khan¹  Muhammad Hamayun¹  Javid Hussain¹  Sang-Mo Kang¹ 

[1]¹School of Applied Biosciences, College of Agriculture and Life Sciences, Kyungpook National University, Daegu 702-701, Korea

关键词: Paraconiothyrium sp.;    ascotoxin;    growth inhibitory effect;    endophytic fungi;   

DOI  :  10.3390/molecules17011103

来源: mdpi

【 摘 要 】

We have isolated five endophytic fungi from the roots of Capsicum annuum, Cucumis sativus and Glycine max. The culture filtrates (CF) of these endophytes were screened on dwarf mutant rice (Waito-C) and normal rice (Dongjin-byeo). Endophyte CAC-1A significantly inhibited the growth of Waito-C and Dongjin-byeo. Endophyte CAC-1A was identified as Paraconiothyrium sp. by sequencing the ITS rDNA region and phylogenetic analysis. The ethyl acetate fraction of Paraconiothyrium sp. suppressed the germination of Lactuca sativa and Echinochloa crus-galli seeds. The ethyl acetate fraction of the endophyte was subjected to bioassay-guided isolation and we obtained the phytotoxic compound ascotoxin (1) which was characterized through NMR and GC/MS techniques. Ascotoxin revealed 100% inhibitory effects on seed germination of Echinochloa crus-galli. Compound (1) was isolated for the first time from Paraconiothyrium sp.

【 授权许可】

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