Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

BMC Microbiology

Thiago Macêdo Lopes Correia¹  Guilherme Barreto Campos¹  Yan Bento dos Anjos¹  Larissa Silva Carvalho Pena¹  Mariluze Peixoto Cruz¹  Regiane Yatsuda¹  Hiago Ferreira Leão¹  Beatriz Almeida Sampaio¹  Mariane Mares Reis¹  Thamara Louisy Santos Brito¹  Carolline Florentino Almeida¹  Laís Ferraz Brito¹  Jorge Timenetsky²  Ana Paula Trovatti Uetanabaro³  Lucas Miranda Marques³  Rachel Passos Rezende³  Carla Cristina Romano³  Andréa Miura da Costa³  Hellen Braga Martins de Oliveira³  Nathan das Neves Selis³ 

[1] Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia;Instituto de Ciências Biomédicas, Universidade de São Paulo;Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Pavilhão Max de Menezes, Universidade Estadual de Santa Cruz;

关键词: L. plantarum;    G. vaginalis;    N. gonorrhoeae;    Cell-free culture supernatant;    Organic acids;    Probiotics;   

DOI  :  10.1186/s12866-021-02264-5

来源: DOAJ

【 摘 要 】

Abstract Background Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. Results The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. Conclusion The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.

【 授权许可】

Unknown