Lactiplantibacillus plantarum strains isolated from spontaneously fermented cocoa exhibit potential probiotic properties against Gardnerella vaginalis and Neisseria gonorrhoeae

BMC Microbiology

article

das Neves Selis, Nathan¹  Brito, Thamara Louisy Santos²  Brito, Laís Ferraz²  Campos, Guilherme Barreto²  Timenetsky, Jorge³  Cruz, Mariluze Peixoto²  Rezende, Rachel Passos¹  Romano, Carla Cristina¹  da Costa, Andréa Miura¹  Yatsuda, Regiane²  Uetanabaro, Ana Paula Trovatti¹  de Oliveira, Hellen Braga Martins¹  Marques, Lucas Miranda¹  Leão, Hiago Ferreira²  dos Anjos, Yan Bento²  Sampaio, Beatriz Almeida²  Correia, Thiago Macêdo Lopes²  Almeida, Carolline Florentino²  Pena, Larissa Silva Carvalho²  Reis, Mariane Mares² 

[1] Programa de Pós-Graduação em Biologia e Biotecnologia de Microrganismos, Universidade Estadual de Santa Cruz;Instituto Multidisciplinar em Saúde, Universidade Federal da Bahia;Instituto de Ciências Biomédicas, Universidade de São Paulo;Departamento de Ciências Biológicas, Laboratório de Microbiologia da Agroindústria, Universidade Estadual de Santa Cruz

关键词: L;    plantarum;    G;    vaginalis;    N;    gonorrhoeae;    Cell-free culture supernatant;    Organic acids;    Probiotics;    Metabolome;   

DOI  :  10.1186/s12866-021-02264-5

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Probiotics are important tools in therapies against vaginal infections and can assist traditional antibiotic therapies in restoring healthy microbiota. Recent research has shown that microorganisms belonging to the genus Lactobacillus have probiotic potential. Thus, this study evaluated the potential in vitro probiotic properties of three strains of Lactiplantibacillus plantarum, isolated during the fermentation of high-quality cocoa, against Gardnerella vaginalis and Neisseria gonorrhoeae. Strains were evaluated for their physiological, safety, and antimicrobial characteristics. The hydrophobicity of L. plantarum strains varied from 26.67 to 91.67%, and their autoaggregation varied from 18.10 to 30.64%. The co-aggregation of L. plantarum strains with G. vaginalis ranged from 14.73 to 16.31%, and from 29.14 to 45.76% with N. gonorrhoeae. All L. plantarum strains could moderately or strongly produce biofilms. L. plantarum strains did not show haemolytic activity and were generally sensitive to the tested antimicrobials. All lactobacillus strains were tolerant to heat and pH resistance tests. All three strains of L. plantarum showed antimicrobial activity against the tested pathogens. The coincubation of L. plantarum strains with pathogens showed that the culture pH remained below 4.5 after 24 h. All cell-free culture supernatants (CFCS) demonstrated activity against the two pathogens tested, and all L. plantarum strains produced hydrogen peroxide. CFCS characterisation in conjunction with gas chromatography revealed that organic acids, especially lactic acid, were responsible for the antimicrobial activity against the pathogens evaluated. The three strains of L. plantarum presented significant probiotic characteristics against the two pathogens of clinical importance. In vitro screening identified strong probiotic candidates for in vivo studies for the treatment of vaginal infections.

【 授权许可】

CC BY|CC0