Production of D-Allose From D-Allulose Using Commercial Immobilized Glucose Isomerase

Frontiers in Bioengineering and Biotechnology

Chang-Su Park¹  Soo-Jin Yeom²  Kyung-Chul Shin³  Yeong-Su Kim⁴  Baek-Joong Kim⁵  Mi Na Choi⁶  Dae Wook Kim⁶ 

[1] D Center, DAESANG Corporation, Icheon, South Korea;Department of Food Science and Technology, Daegu Catholic University, Gyeongsan, South Korea;Department of Integrative Bioscience and Biotechnology, Konkuk University, Seoul, South Korea;School of Biological Sciences and Technology, Chonnam National University, Gwangju, South Korea;;Starch and Sweetener Research Department, Ingredient R&Wild Plants Industrialization Research Division, Baekdudaegan National Arboretum, Bonghwa, South Korea;

关键词: D-allose;    D-allulose;    rare sugar;    packed bed reactor;    Sweetzyme IT;    glucose isomerase;   

DOI  :  10.3389/fbioe.2021.681253

来源: DOAJ

【 摘 要 】

Rare sugars are regarded as functional biological materials due to their potential applications as low-calorie sweeteners, antioxidants, nucleoside analogs, and immunosuppressants. D-Allose is a rare sugar that has attracted substantial attention in recent years, owing to its pharmaceutical activities, but it is still not widely available. To address this limitation, we continuously produced D-allose from D-allulose using a packed bed reactor with commercial glucose isomerase (Sweetzyme IT). The optimal conditions for D-allose production were determined to be pH 8.0 and 60°C, with 500 g/L D-allulose as a substrate at a dilution rate of 0.24/h. Using these optimum conditions, the commercial glucose isomerase produced an average of 150 g/L D-allose over 20 days, with a productivity of 36 g/L/h and a conversion yield of 30%. This is the first report of the successful continuous production of D-allose from D-allulose by commercial glucose isomerase using a packed bed reactor, which can potentially provide a continuous production system for industrial applications of D-allose.

【 授权许可】

Unknown