The Inhibition of miR-144-3p on Cell Proliferation and Metastasis by Targeting TOP2A in HCMV-Positive Glioblastoma Cells

Molecules

Ningning He¹  Dongmeng Qian¹  Bin Wang¹  Jingyi Song¹  Ming Hu¹  Qingxia Ma¹ 

[1] School of Basic Medical Sciences, Qingdao University, 38 Dengzhou Road, Qingdao 266021, China;

关键词: glioma;    HCMV;    miR-144-3p;    Top2A;    proliferation;   

DOI  :  10.3390/molecules23123259

来源: DOAJ

【 摘 要 】

Glioblastoma (GBM), the most common type of primary tumor in the central nervous system, is a very aggressive brain tumor with poor prognosis and a high recurrence rate. Increasing evidence suggests that human cytomegalovirus (HCMV) infection is related to GBM and leads to GBM cell growth and metastasis. MicroRNAs are important regulators in the growth and metastasis of glioblastoma. This study aimed to demonstrate the role of miR-144-3p in HCMV-positive glioblastoma. We found that, after HCMV infection, the expression of miR-144-3p decreased, whereas the expression of TOP2A increased. Bioinformatics analyses indicated that miR-144-3p directly targets the TOP2A 3′-UTR (Untranslated Region). We discovered that the overexpression of miR-144-3p downregulated the overexpression of TOP2A and inhibited the proliferation, clone formation, and invasion of HCMV-positive glioma in vitro. Taken together, these results show that miR-144-3p inhibited growth and promoted apoptosis in glioma cells by targeting TOP2A.

【 授权许可】

Unknown