Pachypodol attenuates Perfluorooctane sulphonate-induced testicular damage by reducing oxidative stress

Saudi Journal of Biological Sciences

Khalid Al-Ghanim¹  S. Mahboob²  Satyanarayana Swamy Mruthinti³  Asma Ashraf⁴  Hussain Ahmed⁵  Ayesha Rauf⁶  Shama Mustafa⁶  Muhammad Umar Ijaz⁶ 

[1] Corresponding author.;Department of Biology, University of West Georgia, Carrollton, GA, USA;Department of Zoology, College of Science, King Saud University, Saudi Arabia;Department of Zoology, Government College University, Faisalabad, Pakistan;Department of Zoology, The University of Buner, Khyber Pakhtunkhwa, Pakistan;Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad, Pakistan;

关键词: Perfluorooctane sulfonate;    Pachypodol;    Testicular toxicity;    Reactive oxygen species;    Oxidative stress;    Antioxidant;   

DOI  :  

来源: DOAJ

【 摘 要 】

Perfluorooctane sulfonate (PFOS) is an endocrine disruptor chemical (EDC) with potentially adverse effects on the male reproductive system. Pachypodol (5,4′-dihydroxy-3,7,3′-trimethoxyflavone) is a promising flavonoid isolated from Pogostemon cablin (Blanco) Benth that shows a broad range of pharmacological properties. However, the potential curative effects of pachypodol on testicular toxicity are not available until now. Therefore, this research was proposed to examine the efficiency of pachypodol against PFOS-induced testicular toxicity in adult male rats. The experiments were conducted on Sprague-Dawley rats (n = 48), which were equally distributed into four groups: control, PFOS (20 mg/kg), PFOS + Pachypodol (20 mg/kg + 10 mg/kg respectively), and Pachypodol (10 mg/kg). After 56 days of treatment, testes were excised by slaughtering rats, weighed, and stored till further analysis. The estimated parameters include biochemical markers, spermatogenic indices, hormonal and histopathological profiles. PFOS exposure disturbed the biochemical profile by altering the antioxidant/oxidant balance. For instance, it decreased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione reductase (GSR) while increasing the concentration of reactive oxygen species (ROS) and level of thiobarbituric acid reactive substances (TBARS). PFOS intoxication also led to a notable decline in viability, motility, epididymal sperm count, and the number of HOS coiled-tail sperms, whereas the higher level of abnormality in the head, mid-piece, and tail of sperms were observed. Besides, it lowered luteinizing hormone (LH), follicle-stimulating hormone (FSH), and plasma testosterone. In addition, PFOS exposure led to histopathological damages in testicles. However, pachypodol treatment potently alleviated all the illustrated impairments in testes. Conclusively, our results demonstrate the promising free-radical scavenging activity of pachypodol, a novel phytochemical, against the PFOS-instigated testicular dysfunctions.

【 授权许可】

Unknown