Overexpression of UGT74E2, an Arabidopsis IBA Glycosyltransferase, Enhances Seed Germination and Modulates Stress Tolerance via ABA Signaling in Rice

International Journal of Molecular Sciences

Pan Li¹  Shanghui Jin²  Chengchao Zheng³  Ting Wang⁴  Tianjiao Mu⁴  Guangrui Dong⁴  Tingting Chen⁴  Yanjie Li⁴  Bingkai Hou⁴ 

[1] College of Pharmacy, Liaocheng University, Liaocheng 252000, China;School of Life Science, Qingdao Agricultural University, Qingdao 266109, China;State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian 271018, China;The Key Laboratory of Plant Development and Environment Adaptation Biology, Ministry of Education, School of Life Science, Shandong University, Qingdao 266237, China;

关键词: UDP-glycosyltransferases;    glycosylation;    indole-3-butyric acid;    abscisic acid;    drought;    salt;   

DOI  :  10.3390/ijms21197239

来源: DOAJ

【 摘 要 】

UDP-glycosyltransferases (UGTs) play key roles in modulating plant development and responses to environmental challenges. Previous research reported that the Arabidopsis UDP-glucosyltransferase 74E2 (AtUGT74E2), which transfers glucose to indole-3-butyric acid (IBA), is involved in regulating plant architecture and stress responses. Here, we show novel and distinct roles of UGT74E2 in rice. We found that overexpression of AtUGT74E2 in rice could enhance seed germination. This effect was also observed in the presence of IBA and abscisic acid (ABA), as well as salt and drought stresses. Further investigation indicated that the overexpression lines had lower levels of free IBA and ABA compared to wild-type plants. Auxin signaling pathway gene expression such as for OsARF and OsGH3 genes, as well as ABA signaling pathway genes OsABI3 and OsABI5, was substantially downregulated in germinating seeds of UGT74E2 overexpression lines. Consistently, due to reduced IBA and ABA levels, the established seedlings were less tolerant to drought and salt stresses. The regulation of rice seed germination and stress tolerance could be attributed to IBA and ABA level alterations, as well as modulation of the auxin/ABA signaling pathways by UGT74E2. The distinct roles of UGT74E2 in rice implied that complex and different molecular regulation networks exist between Arabidopsis and rice.

【 授权许可】

Unknown