The analysis of new higher operative bioactive compounds and chemical functional group from herbal plants through UF-HPLC-DAD and Fourier transform infrared spectroscopy methods and their biological activity with antioxidant potential process as future green chemical assay

Arabian Journal of Chemistry

Rafia Azmat¹  M. Liaquat²  Rasheed Ahmed²  Ayaz Mehmood³  Saima Naz⁴  Waseem Ahmed⁵  Abdul Qayyum⁶  Sami Ullah Khan⁶  Shakil Ahmad⁷ 

[1] Corresponding author.;Department of Agronomy, The University of Haripur, 22620, Pakistan;Department of Chemistry, University of Karachi, University Town, 74700 Karachi, Pakistan;Department of Food Science and Technology, The University of Haripur, 22620, Pakistan;Department of Horticulture, The University of Haripur, Hatatr Road, 22620, Pakistan;Department of Soil Science, The University of Haripur, 22620, Pakistan;Division of Science and Technology Department of Chemistry, University of Education Lahore, Multan Campus, Pakistan;

关键词: Herbal plants;    UF-HPLC-DAD;    ESR;    FTIR;    Bioactive compounds;    DPPH;   

DOI  :  

来源: DOAJ

【 摘 要 】

The two herbal plants of Adhatoda vasica and Calotropis procera, commonly available in worldwide, were selected to explore their medicinal significance. The isolation and quantitative analysis of ten important bioactive compounds such as P-hydroxy-benzoic, Stictic acid, Usnic acid, Epigallocatechin gallate, Quercetin, Epigallocatechin, Catechin, Morin, Epicatechin and Naringenin, isolated from various parts was performed by using UF-HPLC-DAD and FTIR techniques. The antioxidant potentials of isolated bioactive compounds were evaluated through six methods including Electron spin resonance spectrometry, ABTS Assay, Diphenyl picrylhydrazyl (DPPH) radical scavenging assay, total radical-trapping, ORAC, and phycoerythrin assay on various plant parts. The advanced ESR method along with five other methods was applied for the first time on these species to evaluate the antioxidant potential of both species. The results showed that the flowers of Adhatoda vasica prove to be a rich source of important compounds in which Naringenin (15 ± 0.41) was higher in the flowers. The similar trend observed in twigs and seeds of Adhatoda vasica, the Epicatechin was higher in seeds of Calotropis procera. Results showed that the electron spin resonance spectrometry and ABTS radical scavenging assay methods showed higher antioxidant potential in Adhatoda vasica and Calotropis procera as compared to Diphenyl picrylhydrazyl (DPPH) radical scavenging assay.

【 授权许可】

Unknown