Fourier transform infrared spectrum of the radical cation of β‐carotene photoinduced in photosystem II

FEBS Letters

Mitsuka, Tomoya¹  Inoue, Yorinao¹  Noguchi, Takumi¹ 

[1]Solar Energy Research Group, The Institute of Physical and Chemical Research (Riken), Wako, Saitama 351-01, Japan

关键词: Photosynthesis;    Photosystem II;    Fourier transform infrared spectroscopy;    Carotenoid;    Radical cation;    Chl;    accessory chlorophyll of photosystem II;    DCMU;    3-(3;    4-dichlorophenyl)-1;    1-dimethylurea;    ESR;    electron spin resonance;    FTIR;    Fourier infrared;    IR;    infrared;    MES;    2-(N-morpholino)ethanesulfonic acid;    P680;    primary electron donor of photosystem II;    PSII;    photosystem II;    QA;    primary quinone acceptor;    UVVIS-NIR;    ultraviolet-visible-near infrared;   

DOI  :  10.1016/0014-5793(94)01263-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A Fourier-transform infrared (FTIR) spectrum of the radical cation of β-carotene photoinduced in photosystem II (PSII) membranes was obtained at 80K under oxidizing conditions, by utilizing the light-induced FTIR difference technique. Formation of the β-carotene cation was monitored with the electronic absorption band at 993 nm. An FTIR spectrum of a chemically-generated β-carotene cation in chloroform was also measured and compared with the spectrum of PSII. Since the FTIR bands of carotenoid cation have characteristic features with strong intensities, they can be useful markers in studying the reaction of carotenoid in PSII.

【 授权许可】

Unknown