Current status and perspectives of genome editing technology for microalgae

Biotechnology for Biofuels

Byeong-ryool Jeong¹  Seungjib Jeon¹  Yong Keun Chang¹  Nam Kyu Kang¹  Hee-Mock Oh²  Hyung-Gwan Lee²  Youn-Il Park³  Sung-Eun Shin⁴  Won-Joong Jeong⁵  Jong-Min Lim⁵ 

[1] Advanced Biomass Research and Development Center (ABC);Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB);Department of Biological Sciences, Chungnam National University;LG Chem;Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB);

关键词: Genetic engineering;    Microalgae;    Genome editing;    CRISPR/Cas9;    Biofuels;    GMO conflicts;   

DOI  :  10.1186/s13068-017-0957-z

来源: DOAJ

【 摘 要 】

Abstract Genome editing techniques are critical for manipulating genes not only to investigate their functions in biology but also to improve traits for genetic engineering in biotechnology. Genome editing has been greatly facilitated by engineered nucleases, dubbed molecular scissors, including zinc-finger nuclease (ZFN), TAL effector endonuclease (TALEN) and clustered regularly interspaced palindromic sequences (CRISPR)/Cas9. In particular, CRISPR/Cas9 has revolutionized genome editing fields with its simplicity, efficiency and accuracy compared to previous nucleases. CRISPR/Cas9-induced genome editing is being used in numerous organisms including microalgae. Microalgae have been subjected to extensive genetic and biological engineering due to their great potential as sustainable biofuel and chemical feedstocks. However, progress in microalgal engineering is slow mainly due to a lack of a proper transformation toolbox, and the same problem also applies to genome editing techniques. Given these problems, there are a few reports on successful genome editing in microalgae. It is, thus, time to consider the problems and solutions of genome editing in microalgae as well as further applications of this exciting technology for other scientific and engineering purposes.

【 授权许可】

Unknown