Oligonucleotide and Long Polymeric DNA Encoding.

Mariella, R. P. ; Christian, A. T. ; Young, J. A. ; Gardner, S. N. ; Clague, D. S. ; Williams, J. M. ; Miller, E. L.

Technical Information Center Oak Ridge Tennessee

关键词: Deoxyribonucleic acids;    Polymers;    Clones;    Functionals;    Genetic engineering;   

RP-ID  :  DE200415009752

学科分类：工程和技术（综合）

美国|英语

来源: National Technical Reports Library

【 摘 要 】

This report summarizes the work done at Lawrence Livermore National Laboratory for the Oligonucleotide and Long Polymeric DNA Encoding project, part of the Microelectronic Bioprocesses Program at DARPA. The goal of the project was to develop a process by which long (circa 10,000 base-pair) synthetic DNA molecules could be synthesized in a timely and economic manner. During construction of the long molecule, errors in DNA sequence occur during hybridization and/or the subsequent enzymatic process. The work done on this project has resulted in a novel synthesis scheme that we call the parallel pyramid synthesis protocol, the development of a suit of computational tools to minimize and quantify errors in the synthesized DNA sequence, and experimental proof of this technique.