Structural, Surface, in vitro Bacterial Adhesion and Biofilm Formation Analysis of Three Dental Restorative Composites

Materials

Rani Faryal¹  Ihtesham U. Rehman²  Danish Muzzafar³  Saadat A. Siddiqi⁴  Abdul S. Khan⁴  Maria T. Azam⁴  Aqif A. Chauhdry⁴  Riaz Ahmad⁵ 

[1] Department of Biosciences, COMSATS Institute of Information Technology, Islamabad 44000, Pakistan;Department of Materials Science and Engineering, the Kroto Research Institute,the University of Sheffield, Sheffield S3 7HQ, UK;Faculty of Dentistry, SEGi University, Kota Damansara, Petaling Jaya 47810, Malaysia;Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology, Lahore 54000, Pakistan;The Centre for Advanced Studies in Physics, Government College University, Lahore 54000, Pakistan;

关键词: dental composites;    spectroscopic analysis;    bacterial adhesion;    wettability;    surface properties;   

DOI  :  10.3390/ma8063221

来源: DOAJ

【 摘 要 】

This study was conducted to investigate the relationship between dental materials and bacterial adhesion on the grounds of their chemical composition and physical properties. Three commercially available dental restorative materials (Filtek™Z350, Filtek™P90 and Spectrum®TPH®) were structurally analyzed and their wettability and surface roughness were evaluated by using Fourier Transform Infrared Spectroscopy, Contact Angle Measurement and Atomic Force Microscopy, respectively. These materials were molded into discs and tested with three bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia) for microbial attachment. The bacterial adhesion was observed at different time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, along with Colony Forming Unit Count and Optical Density measurement of the media. It was found that all materials showed a degree of conversion with time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, which led to the availability of functional groups (N–H and C–H) that might promote adhesion. The trend in difference in the extent of bacterial adhesion can be related to particle size, chemical composition and surface wettability of the dental materials.

【 授权许可】

Unknown