Structural, Surface, in vitro Bacterial Adhesion and Biofilm Formation Analysis of Three Dental Restorative Composites

Materials

Maria T. Azam¹  Abdul S. Khan¹  Danish Muzzafar²  Rani Faryal⁵  Saadat A. Siddiqi¹  Riaz Ahmad³  Aqif A. Chauhdry¹  Ihtesham U. Rehman⁴ 

[1] Interdisciplinary Research Centre in Biomedical Materials, COMSATS Institute of Information Technology, Lahore 54000, Pakistan; E-Mails:;Faculty of Dentistry, SEGi University, Kota Damansara, Petaling Jaya 47810, Malaysia; E-Mail:;The Centre for Advanced Studies in Physics, Government College University, Lahore 54000, Pakistan; E-Mail:;Department of Materials Science and Engineering, the Kroto Research Institute, the University of Sheffield, Sheffield S3 7HQ, UK; E-Mail:;Department of Biosciences, COMSATS Institute of Information Technology, Islamabad 44000, Pakistan; E-Mail:

关键词: dental composites;    spectroscopic analysis;    bacterial adhesion;    wettability;    surface properties;   

DOI  :  10.3390/ma8063221

来源: mdpi

【 摘 要 】

This study was conducted to investigate the relationship between dental materials and bacterial adhesion on the grounds of their chemical composition and physical properties. Three commercially available dental restorative materials (Filtek™Z350, Filtek™P90 and Spectrum^®TPH^®) were structurally analyzed and their wettability and surface roughness were evaluated by using Fourier Transform Infrared Spectroscopy, Contact Angle Measurement and Atomic Force Microscopy, respectively. These materials were molded into discs and tested with three bacterial strains (Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia) for microbial attachment. The bacterial adhesion was observed at different time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, along with Colony Forming Unit Count and Optical Density measurement of the media. It was found that all materials showed a degree of conversion with time intervals, i.e., 0 h, 8 h, 24 h, 48 h and 72 h, which led to the availability of functional groups (N–H and C–H) that might promote adhesion. The trend in difference in the extent of bacterial adhesion can be related to particle size, chemical composition and surface wettability of the dental materials.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.