Frontiers in Physiology | |
Are the Follicular Fluid Characteristics of Recovered Coronavirus Disease 2019 Patients Different From Those of Vaccinated Women Approaching in vitro Fertilization? | |
Angela Ferri1  Sergio C. A. Schettini1  Paola Colucci1  Assunta Iuliano1  Maria A. Castiglione Morelli2  Angela Ostuni2  Ilenia Matera2  Licia Viggiani2  | |
[1] Center for Reproductive Medicine of “San Carlo” Hospital, Potenza, Italy;Department of Sciences, University of Basilicata, Potenza, Italy; | |
关键词: SARS-CoV-2; COVID-19; follicular fluid; metabolomics; NMR; anti SARS-CoV-2 immunoglobulins; | |
DOI : 10.3389/fphys.2022.840109 | |
来源: DOAJ |
【 摘 要 】
The aim of this pilot study is to evaluate if SARS-CoV-2 infection or vaccination against SARS-CoV-2 infection induce observable metabolic effects in follicular fluid of women who are following in vitro fertilization (IVF) treatments. The possible impact of coronavirus disease 2019 (COVID-19) on fertility and IVF outcome is considered. We have selected for this study: six women vaccinated against SARS-CoV-2 infection, five recovered COVID-19 patients, and we used nine healthy women as the control group. At the time of oocytes retrieval from participants in the study, follicular fluids were collected and metabolomic analysis was performed by 1H NMR spectroscopy in combination with multivariate analysis to interpret the spectral data. The search for antibody positivity in the follicular fluid aspirates was also carried out, together with the western blotting analysis of some inflammatory proteins, interleukin-6, tumor necrosis factor α (TNFα), and the free radical scavenger superoxide dismutase 2. Higher levels of Ala and Pro together with lower levels of lipids and trimethylamine N-oxide (TMAO) were found in follicular fluids (FFs) of vaccinated women while lower levels of many metabolites were detected in FFs of recovered COVID patients. Expression level of TNF-α was significantly lower both in recovered COVID-19 patients and vaccinated women in comparison to healthy controls.
【 授权许可】
Unknown