Proteomic and Metabolomic Signatures Associated With the Immune Response in Healthy Individuals Immunized With an Inactivated SARS-CoV-2 Vaccine

Frontiers in Immunology

Laurence Don Wai Luu¹  Shufang Wang¹  Jun Tai¹  Xiaodai Cui¹  Jieqiong Li²  Xi Chen²  Fu Jin²  Xiong Zhu²  Xiaoxia Wang²  Xiaolan Huang³  Yi Wang³  Xiaocui Zhou³  Licheng Wang⁴  Shaojin Chen⁵ 

[1] Clinical Laboratory of Sanya People’s Hospital, Sanya, China;;Central &Experimental Research Center, Capital Institute of Pediatrics, Beijing, China;Nursing department of Sanya People’s Hospital, Sanya, China;School of Biotechnology and Biomolecular Science, University of New South Wales, Sydney, NSW, Australia;

关键词: COVID-19;    SARS-CoV-2;    CoronaVac;    proteomics;    metabolomics;    immune response.;   

DOI  :  10.3389/fimmu.2022.848961

来源: DOAJ

【 摘 要 】

CoronaVac (Sinovac), an inactivated vaccine for SARS-CoV-2, has been widely used for immunization. However, analysis of the underlying molecular mechanisms driving CoronaVac-induced immunity is still limited. Here, we applied a systems biology approach to understand the mechanisms behind the adaptive immune response to CoronaVac in a cohort of 50 volunteers immunized with 2 doses of CoronaVac. Vaccination with CoronaVac led to an integrated immune response that included several effector arms of the adaptive immune system including specific IgM/IgG, humoral response and other immune response, as well as the innate immune system as shown by complement activation. Metabolites associated with immunity were also identified implicating the role of metabolites in the humoral response, complement activation and other immune response. Networks associated with the TCA cycle and amino acids metabolic pathways, such as phenylalanine metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, and glycine, serine and threonine metabolism were tightly coupled with immunity. Critically, we constructed a multifactorial response network (MRN) to analyze the underlying interactions and compared the signatures affected by CoronaVac immunization and SARS-CoV-2 infection to further identify immune signatures and related metabolic pathways altered by CoronaVac immunization. These results help us to understand the host response to vaccination of CoronaVac and highlight the utility of a systems biology approach in defining molecular correlates of protection to vaccination.

【 授权许可】

Unknown