A Comparative Pilot Study of Bacterial and Fungal Dysbiosis in Neurodevelopmental Disorders and Gastrointestinal Disorders: Commonalities, Specificities and Correlations with Lifestyle

Microorganisms

Ghizlane Bendriss¹  Ibrahim Laswi¹  Ameena Shafiq¹  Krishnadev Pillai¹  Dalia Zakaria¹  Zain Burney¹  Dana Al-Ali¹  Ali Chaari¹  Mohammad Salameh¹  Nada Mhaimeed¹  NohaA. Yousri² 

[1] Premedical Education Department, Weill Cornell Medicine Qatar, Doha 24144, Qatar;Research Department, Weill Cornell Medicine Qatar, Doha 24144, Qatar;

关键词: dysbiosis;    microbiome;    bacteriome;    mycobiome;    neurodevelopmental disorders;    gastrointestinal disorders;   

DOI  :  10.3390/microorganisms9040741

来源: DOAJ

【 摘 要 】

Gastrointestinal disorders (GIDs) are a common comorbidity in patients with neurodevelopmental disorders (NDDs), while anxiety-like behaviors are common among patients with gastrointestinal diseases. It is still unclear as to which microbes differentiate these two groups. This pilot study aims at proposing an answer by exploring both the bacteriome and the mycobiome in a cohort of 55 volunteers with NDD, GID or controls, while accounting for additional variables that are not commonly included such as probiotic intake and diet. Recruited participants answered a questionnaire and provided a stool sample using the Fisherbrand collection kit. Bacterial and fungal DNA was extracted using the Qiagen Stool minikit. Sequencing (16sRNA and ITS) and phylogenetic analyses were performed using the PE300 Illumina Miseq v3 sequencing. Statistical analysis was performed using the R package. Results showed a significant decrease in bacterial alpha diversity in both NDD and GID, but an increased fungal alpha diversity in NDD. Data pointed at a significant bacterial dysbiosis between the three groups, but the mycobiome dysbiosis is more pronounced in NDD than in GID. Fungi seem to be more affected by probiotics, diet and antibiotic exposure and are proposed to be the main key player in differentiation between NDD and GID dybiosis.

【 授权许可】

Unknown