Inhibitory effect of red LED irradiation on fibroblasts and co-culture of adipose-derived mesenchymal stem cells

Heliyon

Camila Andréa de Oliveira¹  Lucas de Oliveira Fujii¹  Thiago Antonio Moretti de Andrade¹  Fernando Russo Costa do Bomfim¹  Daniela Fernanda Dezotti Silva¹  Viviane Theodoro¹  Fernanda Aparecida Sampaio Mendonça¹  Gláucia Maria Tech dos Santos¹  Maria Esméria Corezola do Amaral¹  Andrea Aparecida de Aro¹  Marcelo Augusto Marretto Esquisatto¹  Fernanda Oriani Bortolazzo²  André Luis Bombeiro²  Cristina Pontes Vicente²  Giane Daniela Carneiro²  Leticia Dudri Lucke²  Alexandre Leite Rodrigues de Oliveira²  Vanderlei Salvador Bagnato³ 

[1] Biomedical Sciences Graduate Program, University Center of Herminio Ometto Foundation / FHO, Araras, São Paulo, Brazil;Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil;Institute of Physics of São Carlos, University of São Paulo – USP, São Carlos, São Paulo, Brazil;

关键词: Biological sciences;    Cell biology;    Proteins;    Biomedical engineering;    Molecular biology;    Regenerative medicine;   

DOI  :  

来源: DOAJ

【 摘 要 】

The objective of this study was to evaluate the effects of red Light Emiting Diode (red LED) irradiation on fibroblasts in adipose-derived mesenchymal stem cells (ASC) co-culture on the scratch assay. We hypothesized that red LED irradiation could stimulate paracrine secretion of ASC, contributing to the activation of genes and molecules involved in cell migration and tissue repair. ASC were co-cultured with NIH/3T3 fibroblasts through direct contact and subjected to red LED irradiation (1.45 J/cm2/5min6s) after the scratch assay, during 4 days. Four groups were established: fibroblasts (F), fibroblasts + LED (FL), fibroblasts + ASC (FC) and fibroblasts + LED + ASC (FLC). The analyzes were based on Ctgf and Reck expression, quantification of collagen types I and III, tenomodulin, VEGF, TGF-β1, MMP-2 and MMP-9, as well as viability analysis and cell migration. Higher Ctgf expression was observed in FC compared to F. Group FC presented higher amount of tenomodulin and VEGF in relation to the other groups. In the cell migration analysis, a higher number of cells was observed in the scratched area of the FC group on the 4th day. There were no differences between groups considering cell viability, Reck expression, amount of collagen types I and III, MMP-2 and TGF-β1, whereas TGF-β1 was not detected in the FC group and the MMP-9 in none of the groups. Our hypothesis was not supported by the results because the red LED irradiation decreased the healing response of ASC. An inhibitory effect of the LED irradiation associated with ASC co-culture was observed with reduction of the amount of TGF-β1, VEGF and tenomodulin, possibly involved in the reduced cell migration. In turn, the ASC alone seem to have modulated fibroblast behavior by increasing Ctgf, VEGF and tenomodulin, leading to greater cell migration. In conclusion, red LED and ASC therapy can have independent effects on fibroblast wound healing, but the combination of both does not have a synergistic effect. Therefore, future studies with other parameters of red LED associated with ASC should be tested aiming clinical application for tissue repair.

【 授权许可】

Unknown