Platelet lysate promotes the healing of long-standing diabetic foot ulcers: A report of two cases and in vitro study

Heliyon

Dana Al-Hattab¹  Maram Hasan²  Abdalla Awidi³  Nidal Younes⁴  Salim Khraisha⁵  Hanan Jafar⁶  Mohanad Saleh⁶  Lena Abu Ameereh⁶ 

[1] Corresponding author.;Department of Anatomy and Histology, College of Medicine, University of Jordan, Amman, Jordan;Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia;;Environmental Science &;Laboratory for Nanomedicine, Biological &Cell Therapy Center, University of Jordan, Amman, Jordan;

关键词: Cell biology;    Proteins;    Biomedical engineering;    Diabetes;    Regenerative medicine;    Platelet lysate;   

DOI  :  

来源: DOAJ

【 摘 要 】

Long-standing foot ulcers present a great challenge in diabetes care. Platelet products have been suggested as a possible therapeutic option. However, nor the effect of an injectable form of platelet lysate on the healing of ulcers nor that on primary cells of the epidermis have been studied. In the current study, we present two cases of an ongoing clinical trial showing the positive effect of autologous platelet lysate injected perilesional. Both clinical cases treated with injections of hPL showed complete healing of previously un-healed within 8 weeks of treatment. Further, we describe the in vitro effect of human platelet lysate (hPL) on primary human epidermal keratinocytes (HEK) in terms of chemotaxis, migration and proliferation. In vitro, HEK showed enhanced chemotaxis towards the hPL compared to keratinocyte-defined media (p < 0.0001). Their migration was also stimulated especially at hPL concentration of 10%V/V (p < 0.0001). In contrast, hPL significantly inhibited HEK proliferation measured through MTT assay (p < 0.0001). In conclusion, the findings presented here provide preliminary evidence of an explanatory mechanism for the effect of hPL on primary keratinocytes and therefore of their potential use in a clinical setting. hPL promotes keratinocyte migration and therefore closure of foot ulcers.

【 授权许可】

Unknown