Influence of molecular weight on intracellular antioxidant activity of invasive silver carp (Hypophthalmichthys molitrix) protein hydrolysates

Journal of Functional Foods

Kee-Hong Kim¹  Mario Ferruzzi²  Andrea M. Liceaga³  Fernanda San Martin³  Reuben R. Goforth³  Sravanthi P. Malaypally³ 

[1] Corresponding author. Tel.: [phone];fax: [phone].;Department of Food Science, Purdue University, 745 Agriculture Mall Drive, West Lafayette, IN 47907, USA;

关键词: Protein hydrolysates;    Intracellular antioxidant activity;    Molecular weight;    Peptide sequence;   

DOI  :  

来源: DOAJ

【 摘 要 】

Protein hydrolysates from underutilized silver carp (SPH) were prepared using Flavourzyme (F-15 to F-60) and Alcalase (A-15 to A-60) at 15, 30, 45 and 60 min, respectively. SPH F-30 and A-60 showed promising chemical-based antioxidant activity and were further fractionated according to size to evaluate caco-2 cell based antioxidant activity. F-30 and A-60 peptide fractions with <3 kDa (F-30 < 3, A-60 < 3) showed higher cell-based antioxidant activity under stressed and non-stressed conditions. Further, IC50 values of F-30 < 3 (1–3 mg/mL) was lower than A-60 < 3 fractions (4 to 12 mg/mL), indicating higher cellular antioxidant activity of F-30 < 3 compared to A-60 < 3 under all conditions. The presence of active peptides with desired amino acid sequence in F-30 < 3 compared to A-60 < 3 may have contributed to its higher cellular antioxidant activity. Overall, SPH exhibited antioxidant capacity, hence using an underutilized, invasive fish for environmental and economic gain in the form of promising functional ingredients.

【 授权许可】

Unknown