Characterization of human deoxycytidine kinase correlation with cDNA sequences

FEBS Letters

Bohman, Christina¹  Bergman, Tomas²  Cederlund, Ella²  Eriksson, Staffan¹  Jörnvall, Hans² 

[1] Medical Nobel Institute, Department of Biochemistry I Karolinska Institute, Stockholm, Sweden;Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden

关键词: Deoxycytidine kinase;    Peptide sequence;    Amino acid composition;    cDNA sequence;   

DOI  :  10.1016/0014-5793(91)80332-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Existing data on the structure of human deoxycytidine kinase (dCK) diverge. A monomeric 60 kDa form has been isolated and the cloning of a cDNA coding for 626 amino acids corresponding to a 71 kDa protein has been reported. However, pure dCK isolated from leukemic spleen is a dimer of 30 kDa subunits. Amino acid sequences of peptides from digests of this protein are now presented. None of the peptide structures obtained correspond to the cDNA for the 71 kDa protein, but to a cDNA for a 30.5 kDa dCK recently cloned. Furthermore, homology of the peptide sequences of dCK to parts of thymidine kinases and protein-tyrosine kinases are detected.

【 授权许可】

Unknown