Characterization of an Aerosol-Based Photobioreactor for Cultivation of Phototrophic Biofilms

Life

Dorina Strieth¹  Marianne Volkmar¹  Jonas Kollmen¹  Roland Ulber¹  Judith Stiefelmaier¹  Johannes Robert²  Volkmar Jordan²  Kai Muffler³  Michael Lakatos⁴  Andreas Weber⁵ 

[1] Bioprocess Engineering, University of Kaiserslautern, 67663 Kaiserslautern, Germany;Chemical Engineering, University of Applied Sciences Münster, 48565 Steinfurt, Germany;Department of Biotechnology, Enzyme and Fermentation Technology, Bioprocess Technology and Fundamentals of Microbiology, University of Applied Sciences Bingen, 55411 Bingen, Germany;Integrative Biotechnology, Applied Logistics and Polymer Sciences, University of Applied Sciences Kaiserslautern, 66953 Pirmasens, Germany;Math2Market, 67655 Kaiserslautern, Germany;

关键词: bioengineering;    biofilm;    cyanobacteria;    photosynthesis;    antibiotics;    neurodegenerative disease;   

DOI  :  10.3390/life11101046

来源: DOAJ

【 摘 要 】

Phototrophic biofilms, in particular terrestrial cyanobacteria, offer a variety of biotechnologically interesting products such as natural dyes, antibiotics or dietary supplements. However, phototrophic biofilms are difficult to cultivate in submerged bioreactors. A new generation of biofilm photobioreactors imitates the natural habitat resulting in higher productivity. In this work, an aerosol-based photobioreactor is presented that was characterized for the cultivation of phototrophic biofilms. Experiments and simulation of aerosol distribution showed a uniform aerosol supply to biofilms. Compared to previous prototypes, the growth of the terrestrial cyanobacterium Nostoc sp. could be almost tripled. Different surfaces for biofilm growth were investigated regarding hydrophobicity, contact angle, light- and temperature distribution. Further, the results were successfully simulated. Finally, the growth of Nostoc sp. was investigated on different surfaces and the biofilm thickness was measured noninvasively using optical coherence tomography. It could be shown that the cultivation surface had no influence on biomass production, but did affect biofilm thickness.

【 授权许可】

Unknown